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Development of high-performance nitrile hydratase whole-cell catalyst by automated structure- and sequence-based design and mechanism insights
Nitrile hydratase (NHase) is a metalloenzyme that catalyzes the conversion of nitrile to amide and is widely used in the biocatalysis of bulk...
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Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium
Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative...
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Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase
AbstractAdvanced biotransformation processes typically involve the upstream processing part performed continuously and interlinked tightly with the...
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An artificial multienzyme cascade for the whole-cell synthesis of rare ketoses from glycerol
PurposeIn our previous study, we constructed a one-pot multi-enzyme system for rare ketoses synthesis based on L-rhamnulose-1-phosphate aldolase...
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Rapamycin enhanced the production of 2-phenylethanol during whole-cell bioconversion by yeast
Abstract2-Phenylethanol (2-PE), a higher alcohol with a rose-like odor, has been widely utilized in food, perfume, and beverages. Saccharomyces...
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Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑d‑neuraminic acid
N ‑Acetyl‑ d ‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of...
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Purified lactases versus whole-cell lactases—the winner takes it all
Lactose-free dairy products are in great demand worldwide due to the high prevalence of lactose intolerance. To make lactose-free dairy products,...
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Engineering a Pichia pastoris nitrilase whole cell catalyst through the increased nitrilase gene copy number and co-expressing of ER oxidoreductin 1
1-Cyanocyclohexaneacetic acid (1-CHAA) is a critical intermediate for the synthesis of the antiepileptic agent gabapentin. Previously, our group has...
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Single Cell Atlas: a single-cell multi-omics human cell encyclopedia
Single-cell sequencing datasets are key in biology and medicine for unraveling insights into heterogeneous cell populations with unprecedented...
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Analysis of Cell Growth Kinetics in Suspension and Adherent Types of Cell Lines
Cell growth entails an increase in mass and physical size mostly influenced by physical, biological and chemical environments. As a result, each...
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Biosynthesis of TiO2 Nanoparticles and Their Application as Catalyst in Biodiesel Production
Titanium dioxide nanoparticles (TiO2 NPs) are capturing great interest worldwide with vast applications in various areas. However, their conventional...
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Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations
P2X7 receptors play an important role in cytokine release and immune cell regulation. Their upregulation has been described in inflammatory and...
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A novel in-situ-process technique constructs whole circular cpDNA library
BackgroundThe chloroplast genome (cp genome) is directly related to the study and analysis of molecular phylogeny and evolution of plants in the...
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Effective Fermentation of Sugarcane Bagasse Whole Slurries Using Robust Xylose-Capable Saccharomyces cerevisiae
The pre-treatment of lignocellulose material towards cellulosic bioethanol production releases microbial inhibitors that severely limit the...
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Analysis of pyridine-2-carbaldehyde thiosemicarbazone as an anti-biofouling cathodic agent in microbial fuel cell
AbstractMicrobial fuel cells (MFCs) have gained attention due to their applications in the energy and environmental sectors. However, several...
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Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching
In their natural environment, most cells and tissues are continuously exposed to cyclic mechanical strain. Sensing these stimuli by mechanosensory...
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Impregnating biochar with Fe and Cu by bioleaching for fabricating catalyst to activate H2O2
AbstractBiochar is an excellent support material for heterogeneous catalyst in Fenton reaction. However, fabrication of heterogeneous catalyst...
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Stability of the H-cluster under whole-cell conditions—formation of an Htrans-like state and its reactivity towards oxygen
Hydrogenases are metalloenzymes that catalyze the reversible oxidation of molecular hydrogen into protons and electrons. For this purpose,...
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Efficient production of l-menthyl α-glucopyranoside from l-menthol via whole-cell biotransformation using recombinant Escherichia coli
l- Menthyl α -D-glucopyranoside ( α -MenG) is a glycoside derivative of l -menthol with improved water-solubility and new flavor property as a food...
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Localizing Molecules in Plant Cell Walls Using Fluorescence Microscopy
Autofluorescence of plant tissues can be used as a label-free method to detect a range of phenolic-based cell wall components including lignin,...