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1. Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Crocus sativus L.)

   Several limitations in genetic engineering interventions in saffron exist, hindering the development of genetically modified varieties and the...

   Diksha Kalia, Joel Jose-Santhi, ... Rajesh Kumar Singh in Physiology and Molecular Biology of Plants

   Article 20 May 2024
   

2. A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants

   Background

   Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies...

   Yongliang Liu, Ruiqing Lyu, ... Ling Yuan in Plant Methods

   Article Open access 12 February 2024
   

3. Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

   Background

   Virus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions....

   ** Mo, ... Guo** Wang in Plant Methods

   Article Open access 31 August 2023
   

4. Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors

   As an efficient tool for functional genomics, VIGS (virus-induced gene silencing) has been widely used in reverse and forward genetics to identify...

   Tao Zhou, Laihua Dong, ... Zaifeng Fan in Plant Virology

   Protocol 2022
   

5. Identification and virus-induced gene silencing (VIGS) analysis of methyltransferase affecting tomato (Solanum lycopersicum) fruit ripening

   Main conclusion

   Six methyltransferase genes affecting tomato fruit ripening were identified through genome-wide screening, VIGS assay, and expression...

   Jiaxin **ong, Ye Liu, ... Benzhong Zhu in Planta

   Article 01 April 2024
   

6. Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato

   Unveiling of full genome sequence of tomato demands significant advances of tomato functional genomics. Virus-induced gene silencing (VIGS) is a well...

   Ashish Kumar Singh, Dibyendu Ghosh, Supriya Chakraborty in Plant Gene Silencing

   Protocol 2022
   

7. Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)

   Background

   Although the genome for the allotetraploid bioenergy crop switchgrass ( Panicum virgatum ) has been established, limitations in mutant...

   Kira Tiedge, Janessa Destremps, ... Philipp Zerbe in Plant Methods

   Article Open access 30 May 2022
   

8. Optimization of Virus-induced Phytoene Desaturase (PDS) gene silencing using Tobacco Rattle Virus in Coleus forskohlii

   Virus-induced gene silencing (VIGS) is an effective reverse genetics method used to study the gene function by analysis of plants at phenotype and...

   Archana Bhat, Sahaurti Sharma, Sumit G. Gandhi in Plant Cell, Tissue and Organ Culture (PCTOC)

   Article 15 March 2024
   

9. A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1

   Virus-induced gene silencing (VIGS) is a powerful technology for molecular characterization of gene functions in plants and has the potential to...

   Chunqing Wang, Zhimin Ma, ... Zhen Song in Phytopathology Research

   Article Open access 12 March 2024
   

10. Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato

    Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing...

    Zhengming Wang, **ao Yu Wang, ... David C. Baulcombe in Plant Gametogenesis

    Protocol 2022
    

11. Virus-Induced Gene Silencing in Sorghum Using Brome Mosaic Virus

    Sorghum [Sorghum bicolor (L.) Moench.] is a versatile crop, grown in 30 countries and a food source for nearly 500 million people globally. Although...

    Dharmendra K. Singh, Kirankumar S. Mysore in Plant Gene Silencing

    Protocol 2022
    

12. Virus-Induced Gene Silencing in Wheat and Related Monocot Species

    Advances made in genome sequencing projects and structural genomics are generating large repertoire of candidate genes in plants associated with...

    Vinay Panwar, Kostya Kanyuka in Plant Gene Silencing

    Protocol 2022
    

13. VIGS-Based Gene Silencing for Assessing Mineral Nutrient Acquisition

    Virus-induced gene silencing (VIGS), is a transient gene silencing method for plants, allows rapid and parallel characterization of promising...

    ▪ Akash, Rajat Srivastava, Rahul Kumar in Plant Gene Silencing

    Protocol 2022
    

14. Plant Gene Silencing Methods and Protocols

    This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview...

    Kirankumar S. Mysore, Muthappa Senthil-Kumar in Methods in Molecular Biology

    Book 2022
    

15. Virus Induced Gene Silencing: A Tool to Study Gene Function in Wheat

    Bread wheat is a staple crop for human consumption. Recent technological advances have not only helped in availability of a high quality and...

    Gaganpreet Kaur Dhariwal, Raman Dhariwal, ... André Laroche in Genomics of Cereal Crops

    Protocol 2022
    

16. Construction of monopartite geminivirus-based virus-induced gene silencing (VIGS) vectors using a two-component strategy

    Virus-induced gene silencing (VIGS) is a rapid and efficient tool to elucidate plant gene functions by inserting a target gene fragment into a viral...

    Kikyo Watanabe, Aya Sakane, ... Masashi Ugaki in Journal of General Plant Pathology

    Article 27 August 2021
    

17. Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)

    Water dropwort ( Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals....

    Zhen He, Shuangyu Sheng, ... Liangjun Li in Plant Methods

    Article Open access 11 January 2024
    

18. Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants

    Virus-induced gene silencing (VIGS) is a functional genomics tool to transiently downregulate the expression of target gene(s) by exploiting the...

    Dikki Pedenla Bomzan, Krishna Kumar, ... Dinesh A. Nagegowda in Plant Gene Silencing

    Protocol 2022
    

19. Virus-induced gene silencing shows that LATE FLOWERING plays a role in promoting flower development in soybean

    Virus-induced gene silencing (VIGS) is a useful tool to investigate the role of genes particularly in hard-to-transform plant species via the Agrobacterium...

    Seung Yong Shin, Myuong-Ryoul Park, ... Hyo-Jun Lee in Plant Growth Regulation

    Article Open access 19 September 2022
    

20. A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean

    Background

    Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic...

    Fides Angeli Zaulda, Seung Hyun Yang, ... Feng Qu in Plant Methods

    Article Open access 28 October 2022