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A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants
BackgroundVirus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies...
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A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
Virus-induced gene silencing (VIGS) is a powerful technology for molecular characterization of gene functions in plants and has the potential to...
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Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)
BackgroundVirus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions....
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A highly efficient Agrobacterium-mediated infectious system for Sweet potato leaf curl virus and a deltasatellite-based VIGS vector
Plant virus disease is one of the major threats to the yield and quality of sweetpotato ( Ipomoea batatas [L.] Lam.), the seventh most important food...
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Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Crocus sativus L.)
Several limitations in genetic engineering interventions in saffron exist, hindering the development of genetically modified varieties and the...
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Identification and virus-induced gene silencing (VIGS) analysis of methyltransferase affecting tomato (Solanum lycopersicum) fruit ripening
Main conclusionSix methyltransferase genes affecting tomato fruit ripening were identified through genome-wide screening, VIGS assay, and expression...
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Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)
BackgroundAlthough the genome for the allotetraploid bioenergy crop switchgrass ( Panicum virgatum ) has been established, limitations in mutant...
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Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors
As an efficient tool for functional genomics, VIGS (virus-induced gene silencing) has been widely used in reverse and forward genetics to identify...
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California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting
BackgroundTobacco rattle virus (TRV) based virus-induced gene silencing (VIGS), a widely used functional genomics tool, requires growth temperatures...
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RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications
The availability of protocols for virus-induced gene silencing (VIGS) in rice has opened up an important channel for the elucidation of gene...
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VIGS-Based Gene Silencing for Assessing Mineral Nutrient Acquisition
Virus-induced gene silencing (VIGS), is a transient gene silencing method for plants, allows rapid and parallel characterization of promising...
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Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato
Unveiling of full genome sequence of tomato demands significant advances of tomato functional genomics. Virus-induced gene silencing (VIGS) is a well...
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A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings
BackgroundVirus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant...
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Introduction of Bacillus thuringiensis (Bt) gene does not reduce potassium use efficiency of Bt transgenic cotton (Gossypium hirsutum L.)
BackgroundPotassium (K) deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis ( Bt )...
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An Improved Virus-Induced Gene Silencing (VIGS) System in Zoysiagrass
Virus-induced gene silencing (VIGS) is a powerful RNA-silencing technique for transient gene manipulation and functional verification. However, the...
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Establishment of TRV-mediated Gene Silencing and Application for Elucidating Functions of Anthocyanidin Reductase Gene HcANR in Kenaf (Hibiscus cannabinus L.)
Virus-induced gene silencing (VIGS) is a potent way to study gene function in plants. In this study, a tobacco rattle virus (TRV)-mediated gene...
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Validation of molecular response of tuberization in response to elevated temperature by using a transient Virus Induced Gene Silencing (VIGS) in potato
Temperature plays an important role in potato tuberization. The ideal night temperature for tuber formation is ~17 °C while temperature beyond 22 °C...
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An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector
Main conclusionCaLCuV-based VIGS effectively works in cabbage and contributes to efficient functional genomics research in Brassica crop species.
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Development and application of the TRV-induced gene-silencing system in different Rhododendron species
Rhododendron is one of the top ten traditional flowers in China, with high ornamental and medicinal values. However, molecular research on this...
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Virus-induced gene silencing shows that LATE FLOWERING plays a role in promoting flower development in soybean
Virus-induced gene silencing (VIGS) is a useful tool to investigate the role of genes particularly in hard-to-transform plant species via the Agrobacterium...
