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Quantitative Estimation of Promoter Activity in Cannabis sativa Using Agroinfiltration-Based Transient Gene Expression
To properly assess promoter activity, which is critical for understanding biosynthetic pathways in different plant species, we use...
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An effective transient expression system for gene function identification in Lotus japonicus
Transient transformation is a notably efficient and expeditious method for obtaining foreign gene expression products, making it a prominent avenue...
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A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves
BackgroundOptimization of a highly efficient transient expression system is critical for the study of gene function, particularly in those plants in...
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Validation of expression and cellular localization of AtZAT12 gene deleted an EAR motif in Nicotiana benthamiana using the transient expression system
Expression and cellular localization of recombinant proteins play a key role in identifying protein functions. Plant based transient expression was...
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Fast and Easy Transient Mammalian Cell Expression and Purification of Cytoplasmic Dynein
Recombinant protein expression has been key to studying dynein’s mechanochemistry and structure-function relationship. To gain further insight into...
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Transient Gene Expression in Human Expi293 Cells
Transient gene expression (TGE) is an important tool for generating recombinant proteins in a short period of time. The human cell line HEK293 is...
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Establishment of protoplasts transient expression system in Pinellia ternata (Thunb.) Breit
ObjectiveIn this study, we established an efficient and rapid transient expression system in the protoplasts of Pinellia ternata (Thunb.) Breit. ( P....
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Tomato mosaic virus movement protein enhances the transient expression of recombinant protein in the stem of the tomato plant, Solanum lycopersicum
Plant viral movement proteins (MPs) serve a critical role in facilitating intercellular transport, pivotal for propagating infections from afflicted...
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Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat
Seed dormancy genes typically suppress germination and cell divisionCelldivision. Therefore, overexpressing these genes can negatively affect tissue...
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Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression
The agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type...
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A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass
BackgroundWith the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts,...
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Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System
Protein stability influences many aspects of biology, and measuring their stability in vivo can provide important insights into biological systems....
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Transient Gene Expression in Molecular Farming and Functional Genomics of Tea (Camellia sinensis): A Review
Tea ( Camellia sinensis ) protein has piqued frequent interest because of its possible health benefits. In general, plants are theoretically capable of...
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A versatile, rapid Agrobacterium-mediated transient expression system for functional genomics studies in cannabis seedling
Main conclusionWe have developed and optimized a rapid, versatile Agrobacterium -mediated transient expression system for cannabis seedlings that can...
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The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L
BackgroundSafflower ( Carthamus tinctorius L.) is an important economic crop and a traditional medicinal material rich in flavonoids, which can...
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Biosensors for Inducers of Transient Antibiotic Resistance
The acquisition of an antibiotic resistance phenotype can be due to genetic modifications (heritable) or transient changes in bacterial physiology...
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AGROBEST: A Highly Efficient Agrobacterium-Mediated Transient Expression System in Arabidopsis Seedlings
Agrobacterium-mediated transient transformation for gene expression is a simple and fast method to analyze transgene functions in plants....
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Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper ( Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily...
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Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis
Coffea arabica L. is a crucial crop globally, but its genetic homogeneity leads to its susceptibility to diseases and pests like the coffee berry...
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Efficient leaf mesophyll protoplast isolation and transient gene expression in bermudagrasses (Cynodon spp.)
Bermudagrasses ( Cynodon spp. ) are important warm-season turfgrass species with multiple applications. Although physiological and high-throughput...
