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Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR
The ability to control gene expression in mammalian cells is crucial for safe and efficacious gene therapies and for elucidating gene functions....
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Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells
Here, we present a gene regulation strategy enabling programmable control over eukaryotic translational initiation. By excising the natural...
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An electrogenetic interface to program mammalian gene expression by direct current
Wearable electronic devices are playing a rapidly expanding role in the acquisition of individuals’ health data for personalized medical...
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IL-37 isoform D acts as an inhibitor of soluble ST2 to boost type 2 immune homeostasis in white adipose tissue
White adipose tissue (WAT) homeostasis substantiated by type 2 immunity is indispensable to counteract obesity and metabolic disorders....
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Generation of precision preclinical cancer models using regulated in vivo base editing
Although single-nucleotide variants (SNVs) make up the majority of cancer-associated genetic changes and have been comprehensively catalogued, little...
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Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a
AbstractGenome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies...
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Oschib1 gene encoding a GH18 chitinase confers resistance against sheath blight disease of rice caused by Rhizoctonia solani AG1-IA
Sheath blight disease of rice caused by Rhizoctonia solani AG1-IA, is a major fungal disease responsible for huge loss to grain yield and quality....
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Metabolic response to a heterologous poly-3-hydroxybutyrate (PHB) pathway in Phaeodactylum tricornutum
AbstractThe marine diatom Phaeodactylum tricornutum is an emerging host for metabolic engineering, but little is known about how introduced pathways...
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Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens)
Obtaining data on transgene copy number is an integral step in the generation of transgenic plants. Techniques such as Southern blot, segregation...
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Design and Production of Heart Chamber-Specific AAV9 Vectors
Adeno-associated virus serotype 9 (AAV9) is often used in heart research involving gene delivery due to its cardiotropism, high transduction...
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RNA-basierte Regulation der Genexpression: künstliche Genschalter
RNA-based gene control mechanisms pose an elegant and straightforward way to switch on, off, or fine-tune transgene expression without the need for...
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The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants
BackgroundExpansins are cell wall proteins loosening plant cell in pH-dependent manner. This study aimed to investigate the role of AtEXPA18 in...
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Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica
Weed control can be most effectively achieved through the use of herbicide-resistance transgene. A preliminary study bar -transgenic Zoysia japonica ...
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Multiple distinct domains of human XIST are required to coordinate gene silencing and subsequent heterochromatin formation
BackgroundMammalian dosage compensation is achieved by the inactivation of one X chromosome in XX individuals. In eutheria this process is initiated...
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SHP-1 Protein Tyrosine Phosphatase Affects Early Postnatal Bone Development in Mice
The Src homology region 2 domain-containing phosphatase-1 (SHP-1) is an intracellular tyrosine phosphatase that plays a negative regulatory role in...
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Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis
The current coronavirus disease (COVID-19) pandemic remains one of the most serious public health problems. Increasing evidence shows that infection...
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Cre-mediated autoexcision of selectable marker genes in soybean, cotton, canola and maize transgenic plants
Key messageEfficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre...
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Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter
BackgroundTraditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve...
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Um34 in selenocysteine tRNA is required for the expression of stress-related selenoproteins in mammals
Selenium is an essential micronutrient in the diet of mammals and has many health benefits. Selenium-containing proteins are responsible for most, if...
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Genome Editing and Transgenes in Pigs
Disruption of specific target genes is a first step to uncover the gene function. Large-sized animals are difficult to be targeted in embryo level,...