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Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens
Protonemata of the moss Physcomitrium patens are ideal structures in which to observe cytoskeletal organization and dynamics. Special care is needed...
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The cryo-EM 3D image reconstruction of isolated Lethocerus indicus Z-discs
The Z-disk of striated muscle defines the ends of the sarcomere, which repeats many times within the muscle fiber. Here we report application of...
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Careproctus rhomboides, a new snailfish (Cottoidei: Liparidae) from the western North Pacific
The new snailfish Careproctus rhomboides is described on the basis of four specimens collected from the southern Sea of Okhotsk (570–825 m depth),...
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Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function
BackgroundNebulin is a critical thin filament-binding protein that spans from the Z-disk of the skeletal muscle sarcomere to near the pointed end of...
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Fast myosin binding protein C knockout in skeletal muscle alters length-dependent activation and myofilament structure
In striated muscle, the sarcomeric protein myosin-binding protein-C (MyBP-C) is bound to the myosin thick filament and is predicted to stabilize...
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Identification of Toxic Blooms of Cyanobacteria in Freshwater (Lake, Ponds) Habitat
Cyanobacteria are blue-green algae that can develop a massive population to form thick biomass at the surface and outcompete other algae. The massive...
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High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking
A major goal of structural biologists is to preserve samples as close to their living state as possible. High-pressure freezing (HPF) is a...
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Habitat: Muddy Grounds
Deep muddy grounds are the biggest marine habitats in terms of surface area. They cover 90% of the oceans’ floors and start where steep rocky...
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Circulation and Blood
The circulatory system of zebrafish includes heart, arteries, veins and capillaries. The heart is behind the last pair of gills and consists of a...
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Contributions to the palynological study of selected species of Cissus L. (Vitaceae): a new appreciation of ornamentation
Cissus is the largest genus of Vitaceae, with about 300 species widely distributed in tropical regions and temperate areas. Currently, the genus is...
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Formanilides from the culture broth of Perenniporia fraxinea
A new formanilide dimer, fraxinin ( 1 ), and three known formanilides ( 2‒4 ) were isolated from the culture broth of Perenniporia fraxinea using silica...
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Investigation of antimicrobial resistance patterns and molecular ty** of Pseudomonas aeruginosa isolates among Coronavirus disease-19 patients
BackgroundPseudomonas aeruginosa is a common co-infecting pathogen recognized among COVID-19 patients. We aimed to investigate the antimicrobial...
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Morphological development of laboratory-reared Crystallichthys matsushimae (Cottoidei: Liparidae)
Ontogenetic development in the snailfish Crystallichthys matsushimae (Jordan and Snyder 1902), including body proportions, pigmentation, tooth...
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The functional anatomy of elephant trunk whiskers
Behavior and innervation suggest a high tactile sensitivity of elephant trunks. To clarify the tactile trunk periphery we studied whiskers with the...
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Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana
Properly patterned cell walls specify cellular functions in plants. Differentiating protoxylem and metaxylem vessel cells exhibit thick secondary...
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Disruption of Drosophila larval muscle structure and function by UNC45 knockdown
BackgroundProper muscle function is heavily dependent on highly ordered protein complexes. UNC45 is a USC (named since this region is shared by three...
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Intraspecific variations in species of Ophiuroidea (Echinodermata) from the Tropical Southwest Atlantic, Northeast Brazil
Class Ophiuroidea groups the most diverse and abundant organisms of the phylum Echinodermata, having approximately 2100 described species. They have...
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Rearing and Conservation of Nematode Populations Under Greenhouse or Axenic Conditions
Once a nematode has been identified, to conduct studies for screening programs or pathogenicity tests, it is necessary a supply of large numbers of...
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Wood and Wood Density
The density of wood is determined by the relative proportions of its components: vesselsVessels, fibers, and parenchyma cellsParenchyma cells....
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Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy
Peptidoglycan (PG) is an essential component of the bacterial cell wall that protects the cell from turgor pressure and maintains its shape. In...