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Targeted Mutagenesis
Mutational analysis in forward genetics is an effective strategy in invertebrate model animals and zebrafish in vertebrates. Forward genetic studies...
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Targeted knockout of a conserved plant mitochondrial gene by genome editing
Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as genome editing tools for mitochondria. TALE nucleases...
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Exploring genetic diversity and variation of Ovar-DRB1 gene in Sudan Desert Sheep using targeted next-generation sequencing
IntroductionThe Ovar-DRB1 gene, a crucial element of the Major Histocompatibility Complex (MHC) Class II region, initiates adaptive immunity by...
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De novo assembly of transcriptomes and differential gene expression analysis using short-read data from emerging model organisms – a brief guide
Many questions in biology benefit greatly from the use of a variety of model systems. High-throughput sequencing methods have been a triumph in the...
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The multi-faceted roles of R2TP complex span across regulation of gene expression, translation, and protein functional assembly
Macromolecular complexes play essential roles in various cellular processes. The assembly of macromolecular assemblies within the cell must overcome...
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Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using “2A” Self-Cleaving Peptides
Simultaneous stoichiometric expression of multiple genes plays a major part in modern research and biotechnology. Traditional methods for...
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An improved genome assembly of Chrysanthemum nankingense reveals expansion and functional diversification of terpene synthase gene family
BackgroundTerpenes are important components of plant aromas, and terpene synthases (TPSs) are the key enzymes driving terpene diversification. In...
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Chromosome-scale assembly and gene editing of Solanum americanum genome reveals the basis for thermotolerance and fruit anthocyanin composition
Solanum americanum serves as a promising source of resistance genes against potato late blight and is considered as a leafy vegetable for...
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Targeted Gene Editing of Nuclear-Encoded Plastid Proteins in Phaeodactylum tricornutum via CRISPR/Cas9
Genome modifications in microalgae have emerged as a crucial and indispensable tool for research in fundamental and applied biology. In particular,...
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Assembly, Annotation and Visualization of NGS Data
The next-generation sequencing technologies have revolutionized the field of agriculture with the advent of ever-evolving chemistries and a reduction...
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Targeted genome editing for cotton improvement: prospects and challenges
Cotton is a highly profitable agricultural crop globally, valued for its valuable natural cellulosic fibres, renewable biofuel potential, and rich...
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Systematic Analysis of Assembly Intermediates in Yeast to Decipher the Mitoribosome Assembly Pathway
Studies of yeast mitoribosome assembly have been historically hampered by the difficulty of generating mitoribosome protein-coding gene deletion...
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Co-opting the E3 ligase KLHDC2 for targeted protein degradation by small molecules
Targeted protein degradation (TPD) by PROTAC (proteolysis-targeting chimera) and molecular glue small molecules is an emerging therapeutic strategy....
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Efficient Targeted DNA Methylation with dCas9-Coupled DNMT3A-DNMT3L Methyltransferase
Epigenome editing is a powerful approach for the establishment of a chromatin environment with desired properties at a selected genomic locus, which...
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Bioinformatics in Gene and Genome Analysis
Gene and genome analysis play important roles in molecular biology research and individualized medicine. Thanks to the development of sequencing...
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High-efficiency targeted transgene integration via primed micro-homologues
Due to the difficulties in precisely manipulating DNA repair pathways, high-fidelity targeted integration of large transgenes triggered by...
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Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells
Genome editing technologies can be diverted into artificial transcription activators. In particular, researchers have improved dCas9-based...
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Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum
Characterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been...
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Development of super-specific epigenome editing by targeted allele-specific DNA methylation
BackgroundEpigenome editing refers to the targeted reprogramming of genomic loci using an EpiEditor which may consist of an sgRNA/dCas9 complex that...
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A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated...