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Single-Cell Imaging of mRNA by Target RNA-Initiated RCA
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging...
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MiREx: mRNA levels prediction from gene sequence and miRNA target knowledge
Messenger RNA (mRNA) has an essential role in the protein production process. Predicting mRNA expression levels accurately is crucial for...
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mRNA Vaccine Platform: mRNA Production and Delivery
Abstract—Vaccination is the most efficient way to prevent infectious diseases. mRNA-based vaccines is a new approach to vaccine development, which...
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Tethered mRNA Amplifier: A Novel Approach to Increase Protein Expression
Posttranscriptional RNA modification has become a revolutionary clinical tool to improve the underlying condition in genetic disorders. The cell...
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Investigation of sRNA-mRNA Interactions in Bacillus subtilis In Vivo
In this chapter, we describe in vivo methods for the analysis of interactions between an sRNA and its target mRNA in B. subtilis. All these methods...
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mirTarRnaSeq: An R/Bioconductor Statistical Package for miRNA-mRNA Target Identification and Interaction Analysis
We introduce mirTarRnaSeq , an R/Bioconductor package for quantitative assessment of miRNA-mRNA relationships within sample cohorts. mirTarRnaSeq is a...
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Measuring Proximity-Mediated Function of mRNA Regulatory Proteins by Engineered Tethering
A powerful approach for studying the functional consequences of site-specific RNA–protein interactions is to artificially tether a protein to a...
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In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis
So far, in Bacillus subtilis, only four trans-encoded and 11 cis-encoded sRNAs and their targets have been investigated in detail, the majority of...
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High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants
RNA-fluorescence in situ hybridization (RNA-FISH) is an essential and widely used tool for visualizing RNA molecules in intact cells. Recent advances...
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mRNA-encoded Cas13 can be used to treat dengue infections in mice
Dengue is a major global health threat, and there are no approved antiviral agents. Prior research using Cas13 only demonstrated dengue mitigation in...
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mRNA biotherapeutics landscape for rare genetic disorders
The medical emergency of COVID-19 brought to the forefront mRNA vaccine technology where the mRNA vaccine candidates mRNA-1273 and BNT162b2 displayed...
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Effect of nonsense-mediated mRNA decay factor SMG9 deficiency on premature aging in zebrafish
SMG9 is an essential component of the nonsense-mediated mRNA decay (NMD) machinery, a quality control mechanism that selectively degrades aberrant...
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RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
For the past 30 years, in vitro transcription (IVT) technology has been extensively used for RNA production or for basic transcriptional mechanism...
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mRNA Transfection of S. mediterranea for Luminescence Analysis
Planarians have become a powerful model system for stem cell research and regeneration. While the tool kit for mechanistic investigations has been...
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FISH-TAMB, a Fixation-Free mRNA Fluorescent Labeling Technique to Target Transcriptionally Active Members in Microbial Communities
Keystone species or ecological engineers are vital to the health of an ecosystem; however, often, their low abundance or biomass present challenges...
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GraphTar: applying word2vec and graph neural networks to miRNA target prediction
BackgroundMicroRNAs (miRNAs) are short, non-coding RNA molecules that regulate gene expression by binding to specific mRNAs, inhibiting their...
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Application of Repetitive Sequences in Fish Cell Depletion as a Target for the CRISPR/Cas9 System
Specific cell depletion is a common means to study the physiological function of cell lineages and tissue regeneration. However, 100% depletion is...
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Branched chemically modified poly(A) tails enhance the translation capacity of mRNA
Although messenger RNA (mRNA) has proved effective as a vaccine, its potential as a general therapeutic modality is limited by its instability and...
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Comprehensive analysis of miRNA-mRNA regulatory pairs associated with colorectal cancer and the role in tumor immunity
BackgroundMicroRNA (miRNA) which can act as post-transcriptional regulators of mRNAs via base-pairing with complementary sequences within mRNAs is...
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Comparative effectiveness of third doses of mRNA-based COVID-19 vaccines in US veterans
Vaccination against SARS-CoV-2 has been effective in reducing the burden of severe disease and death from COVID-19. Third doses of mRNA-based...
