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Yeast Surface Display
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning...
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A novel, non-GMO surface display in Limosilactobacillus fermentum mediated by cell surface hydrolase without anchor motif
Recent studies have demonstrated the potential of surface display technology in therapeutic development and enzyme immobilization. Utilization of...
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Construction of Semisynthetic Shark vNAR Yeast Surface Display Antibody Libraries
The adaptive immune system of sharks comprises a unique heavy chain-only antibody isotype, termed immunoglobulin new antigen receptor (IgNAR), in...
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The flexible linker and CotG were more effective for the spore surface display of keratinase KERQ7
Feather, horn, hoof, and other keratin waste are protein-rich but limited by natural keratinase synthesis, activity, pH, and temperature stability....
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Display of Oligo-α-1,6-Glycosidase from Exiguobacterium sibiricum on the Surface of Escherichia coli Cells
AbstractCell-surface display using anchor motifs of outer membrane proteins allows exposure of target peptides and proteins on the surface of...
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Antibody Phage Display
The application of antibodies has transcended across many areas of work but mainly as a research tool, for diagnostic and for therapeutic...
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Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display
The majority of therapeutic antibodies, bispecific antibodies, and chimeric antigen receptor (CAR) T cells in cancer therapy are based on an antibody...
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Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display
Yeast surface display (YSD) has been extensively used for protein design, engineering, and directed evolution in the past two decades. Here, we...
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Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening
The binding pocket of some therapeutic targets can acquire multiple conformations that, to some extent, depend on the protein dynamics and the...
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Map** Epitopes by Phage Display
Monoclonal antibodies (mAbs) are valuable biological molecules, serving for many applications. Therefore, it is advantageous to know the interaction...
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Biomarker Discovery by ORFeome Phage Display
Phage display is an efficient and robust method for protein-protein interaction studies. Although it is mostly used for antibody generation, it can...
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Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Yeast surface display has proven to be a powerful tool for the discovery of antibodies and other novel binding proteins and for engineering the...
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Surface Display of Duck Hepatitis A Virus Type 1 VP1 Protein on Bacillus subtilis Spores Elicits Specific Systemic and Mucosal Immune Responses on Mice
Duck viral hepatitis, primarily caused by duck hepatitis A virus type 1 (DHAV-1), poses a significant threat to the global duck industry. Bacillus...
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Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells. This includes typical ECM structures such as collagen and...
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Humanization of Chicken-Derived Antibodies by Yeast Surface Display
Chicken-derived antibodies emerged as a promising tool for diagnostic and therapeutic usage. Due to the phylogenetic distance between birds and...
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Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display
This protocol describes the workflow to isolate and engineer fluorescence-activating proteins by yeast surface display. Fluorescence-activating...
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Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions
The yeast surface display system is a valuable platform for constructing cells with novel functions for various applications and high-throughput...
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Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display
Cattle are known for their repertoire of antibodies harboring extremely long CDR3H regions that form extensive “knob on stalk” cysteine-rich...
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Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Yeast surface display is a powerful directed evolution method for develo** and engineering protein molecules to attain desired properties. Here,...
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Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display
In recent years, the development of bispecific antibodies (bsAbs) has experienced tremendous progress for disease treatment, and consequently, a...