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Isoform-specific RNA structure determination using Nano-DMS-MaP
RNA structure determination is essential to understand how RNA carries out its diverse biological functions. In cells, RNA isoforms are readily...
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All-atom RNA structure determination from cryo-EM maps
Many methods exist for determining protein structures from cryogenic electron microscopy maps, but this remains challenging for RNA structures. Here...
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Data-driven regularization lowers the size barrier of cryo-EM structure determination
Macromolecular structure determination by electron cryo-microscopy (cryo-EM) is limited by the alignment of noisy images of individual particles....
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Cryo-EM for Structure Determination of Mitochondrial Ribosome Samples
Single-particle cryoelectron microscopy (cryo-EM) allows structure determination of large macromolecular complexes from conformationally and...
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Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins
Surface proteins and pili (or pilus) anchored on the Gram-positive bacterial cell wall play a vital role in adhesion, colonization, biofilm...
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AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination
Artificial intelligence-based protein structure prediction methods such as AlphaFold have revolutionized structural biology. The accuracies of these...
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Advances and opportunities in RNA structure experimental determination and computational modeling
Beyond transferring genetic information, RNAs are molecules with diverse functions that include catalyzing biochemical reactions and regulating gene...
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Structure determination of inactive-state GPCRs with a universal nanobody
Cryogenic electron microscopy (cryo-EM) has widened the field of structure-based drug discovery by allowing for routine determination of membrane...
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Single-crystal structure determination of nanosized metal–organic frameworks by three-dimensional electron diffraction
Metal–organic frameworks (MOFs) have attracted considerable interest due to their well-defined pore architecture and structural tunability on...
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In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography
Bacterial surface nanomachines are often refractory to structural determination in their intact form due to their extensive association with the cell...
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Integrative structure determination reveals functional global flexibility for an ultra-multimodular arabinanase
AbnA is an extracellular GH43 α-L-arabinanase from Geobacillus stearothermophilus , a key bacterial enzyme in the degradation and utilization of...
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Structure determination needs to go viral
Viral diseases are expected to cause new epidemics in the future, therefore, it is essential to assess how viral diversity is represented in terms of...
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Nano-DMS-MaP allows isoform-specific RNA structure determination
Genome-wide measurements of RNA structure can be obtained using reagents that react with unpaired bases, leading to adducts that can be identified by...
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Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR
Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique to solve the structure of biomolecular complexes at atomic resolution in...
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Validated determination of NRG1 Ig-like domain structure by mass spectrometry coupled with computational modeling
High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple...
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Direct Determination of the Structure of Single Biopolymer Molecules Using Nanopore Sequencing
AbstractThis review highlights operational principles, features, and modern aspects of the development of third-generation sequencing technology of...
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Stabilization and structure determination of integral membrane proteins by termini restraining
Integral membrane proteins isolated from cellular environment often lose activity and native conformation required for functional analyses and...
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Determination of Histone Methyltransferase Structure by Crystallography
As discussed in previous chapters, the methylation of specific arginine and lysine side chains is carried out by two families of histone...
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The Molecular and Genetic Mechanisms of Sex Determination in Poplar
Abstract —The study of molecular and genetic mechanisms of sex determination in the poplar is of interest not only in the fundamental science, but...
