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Staining procedure to identify paddy with gaps vulnerable to Stenotus rubrovittatus and Trigonotylus caelestialium (Hemiptera: Miridae)
Stenotus rubrovittatus (Matsumura) and Trigonotylus caelestialium (Kirkaldy) (Hemiptera: Miridae) are major rice pests in Japan. Since both species...
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Supported Immunohistochemical Staining of Mucins
Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a...
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Alcian Blue Staining for Chondrocranium Development in Zebrafish
Craniofacial abnormalities are one of the most frequent birth malformations in humans, affecting around one in every thousand live births. The...
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Protargol Staining Technique for Identification of Soil Protists
Protargol staining technique is an excellent way to view the cellular organs of protists such as nuclei, cytostome, cilia, and flagella and to...
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Improvement of gram staining effect by ethanol pretreatment and quantization of staining image by unsupervised machine learning
In this study, we propose an Ethanol Pretreatment Gram staining method that significantly enhances the color contrast of the stain, thereby improving...
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Staining Technology and Bright-Field Microscope Use
We will introduce bright-field microscope use and practice Gram staining with foodborne pathogens and endospore staining with Bacillus cereus.
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SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM)....
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Prussian Blue Staining to Visualize Iron Oxide Nanoparticles
Iron deposits in cells and tissues can be detected by ex vivo histological examination through the Prussian blue (PB) staining. This practical,...
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Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membrane
Mucins are often stained with the basic dye Alcian blue, but mucins with a low acidic glycan content cannot be stained with it. Succinylation-Alcian...
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Advances in AMF Research: Isolation, Histochemical Staining, Enumeration, Morphological and Molecular Techniques
The mutualistic association of arbuscular mycorrhizal fungi (AMF) is an important symbiotic association between the roots of plant and fungi that...
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Phalloidin Staining for F-Actin in Hepatic Stellate Cells
During the development of liver fibrosis, hepatic stellate cells undergo a transition from a quiescent phenotype into a proliferative, fibrogenic,...
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CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, an Alternative to LYMESPOT
Intracellular cytokine staining is a versatile technique used to analyze cytokine production in individual cells by flow cytometry. This methodology...
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Nontraditional Method for Telomere Staining by PNA Probes
The standard FISH uses DNA probes to hybridize to the designated complementary strands. This is DNA-DNA interaction, and it usually takes much longer...
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Staining Methods for Normal and Regenerative Myelin in the Nervous System
Histochemical and fluorescence-based techniques enable the specific identification of myelin by bright-field or fluorescence microscopy. In this...
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Staining to machine learning: An emerging technology for determination of microalgal cell viability
Microalgae are unicellular photosynthetic microorganisms typically found in aquatic environments and they play a vital role in the global carbon and...
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Uranyl-Free Staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy
Uranyl acetate solution has widely been used as staining reagent for samples processed for ultrastructural morphology, cytochemistry, and...
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Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy
Morphological characteristics of liposomes, such as size and lamellarity directly impact their quality and biological performance of encapsulated...
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Co-staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-Seq
The paired detection of the transcriptome and proteome at single-cell resolution provides exquisite insight to immune mechanisms in health and...
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Antibody Staining for Nematodes with Heat-induced Antigen Retrieval (HIAR)
Immunocytochemistry remains a valuable and necessary tool for biologists working with nematodes, even those nematode model organisms with advanced...
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High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain...
