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Differential Proteomic Analysis of Complex Mixtures by Label-Free nLC MS/MS
After over two decades of constant evolution, proteomics can be truly considered nowadays as a high-throughput technique. Latest advances performed...
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Proteomic Analysis of Human Neural Stem Cell Differentiation by SWATH-MS
The unique properties of stem cells to self-renew and differentiate hold great promise in disease modelling and regenerative medicine. However, more...
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Protein biomarker elucidation for the verification of biological agents in the taxonomic group of Gammaproteobacteria using tandem mass spectrometry
Some pathogenic microbes can be used for nefarious applications and instigate population-based fear. In a bio-threat scenario, rapid and accurate...
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Cross-Linking Mass Spectrometry Data Analysis
In this chapter, we will review mass spectrometry-based proteomics and focus on the development of data analysis methods in chemical cross-linking...
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What Can MS, NMR, and TLC Tell Us About the Composition of Lipid Membranes?
Membranes protect the cells against the outer environment but are also very important to enable directed transport inside and outside the cell. In...
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Media Optimization for Direct Somatic Embryogenesis, Regeneration in Sugarcane and Genetic Transformation using cry1Ac Gene
Sugarcane, an important agro-industrial crop, has narrow genetic base, low fertility and is difficult to cross, making crop improvement through...
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Comparative whole-genome and proteomics analyses of the next seed bank and the original master seed bank of MucoRice-CTB 51A line, a rice-based oral cholera vaccine
BackgroundWe have previously developed a rice-based oral vaccine against cholera diarrhea, MucoRice-CTB. Using Agrobacterium -mediated...
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SWATH-MS-facilitated proteomic profiling of fruit skin between Fuji apple and a red skin bud sport mutant
BackgroundApple is one of the most popular fruit crops world-wide and its skin color is an important quality consideration essential for commercial...
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Genetic architecture of protein expression and its regulation in the mouse brain
BackgroundNatural variation in protein expression is common in all organisms and contributes to phenotypic differences among individuals. While...
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Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment
BackgroundThe low efficiency of genetic transformation in Chinese cabbage ( Brassica rapa L . ssp. pekinensis ) is the key problem affecting functional...
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Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome
The dogma “One gene, one protein” is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated...
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Identification of Potential Peptide Inhibitors of ACE-2 Target of SARS-CoV-2 from Buckwheat & Quinoa
It is well established fact that peptides from various foods offer human health benefits displaying diverse functionalities. Millets considered as...
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Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.)
The crop species barley is a genetic model for the small grain temperate cereals. Thanks to the availability of whole genome sequence and the...
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Proteomics for Tea Plant
Plant proteome is highly dynamic and is essential for the investigation of protein expression, interactions, and modifications to better understand...
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Transformation or Genetic Modification of Cells/Organisms
Genetic engineering technology has been employed for a long time to enhance the quantity and quality of a product and produce the desired product...
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Lactic acid bacteria in some Indian fermented foods and their predictive functional profiles
Lactic acid bacteria (LAB) were isolated from naturally fermented foods of India, viz., sidra , a dried fish product; kinema , a naturally fermented...
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An In Silico Outlook for the Detection and Surveillance of Evolving and Persistent Plant Pathogens
The diversity and social behaviour of plants are astounding. They developed a wide variety of molecular mechanisms to react to a complex network of...
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Metabolomics and Cytoplasmic Genomics of Allium
Allium is the biggest genus of petaloid monocotyledons, with more than 750 species widely distributed in a range of climatic conditions worldwide,...
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Quantification of Cell-to-Cell Connectivity Using Particle Bombardment
Plant cells are connected by cytoplasmic bridges called plasmodesmata. Plasmodesmata are lined by the plasma membrane, essentially forming tunnels...
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Metabolomics and Cytoplasmic Genomics of Allium
Allium is the biggest genus of petaloid monocotyledons, with more than 750 species widely distributed in a range of climatic conditions worldwide,...