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Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16S rRNA amplicon sequencing
BackgroundSequential membrane filtration as a pre-processing step for capturing sediment-associated microorganisms could provide good quality and...
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Tethering proteins in membrane traffic
In eukaryotic cells, protein secretion and the transport of materials between membrane-bounded organelles require efficient and accurate delivery of...
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ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
Protein-protein interactions (PPIs) play fundamental roles in all cellular processes. Especially membrane proteins facilitate a range of important...
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Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform
Extracellular vesicles (EVs) are cell-derived membranous structures carrying transmembrane proteins and luminal cargo. Their complex cargo requires...
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Pilot-scale concentration and partial purification of food-grade phycocyanin from Arthrospira platensis via cross flow filtration: From biomass to final product
From food- to analytical-grade products, the commercial exploitation of phycocyanin (PC) strongly depends on its degree of purity. In this study, we...
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Enhancement of α-galactosidase production using novel Actinoplanes utahensis B1 strain: sequential optimization and purification of enzyme
α-Galactosidase is an important exoglycosidase belonging to the hydrolase class of enzymes, which has therapeutic and industrial potential. It plays...
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Influence of Membrane Separation Processes on Food Bioactives
Membrane separation technology is a valuable tool that could be applied in food technology for different purposes. In fact, the membrane separation...
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Phosphatidylserine-dependent structure of synaptogyrin remodels the synaptic vesicle membrane
Synaptic vesicles are small membrane-enclosed organelles that store neurotransmitters at presynaptic terminals. The uniform morphology of synaptic...
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Formation of Amyloid-Like Conformational States of β-Structured Membrane Proteins on the Example of OMPF Porin from the Yersinia pseudotuberculosis Outer Membrane
AbstractThe work presents results of the in vitro and in silico study of formation of amyloid-like structures under harsh denaturing conditions by...
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Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane
Defects in plasma membrane repair can lead to muscle and heart diseases in humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53)...
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Potent and broadly neutralizing antibodies against sarbecoviruses induced by sequential COVID-19 vaccination
The current SARS-CoV-2 variants strikingly evade all authorized monoclonal antibodies and threaten the efficacy of serum-neutralizing activity...
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Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins
Superparamagnetic nanobeads offer several advantages over microbeads for immunocapture of nanocarriers (extracellular vesicles, lipoproteins, and...
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Sequential interspecies interactions affect production of antimicrobial secondary metabolites in Pseudomonas protegens DTU9.1
Soil and rhizosphere microbiomes play important roles in suppression of plant pathogens through production of antagonistic secondary metabolites, yet...
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Structure, dynamics and assembly of the ankyrin complex on human red blood cell membrane
The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and...
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High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications
Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face...
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Mechanically stirred enzymatic membrane reactor containing HRP immobilized on Tau-SiO2@Fe3O4-GO nanocomposite for removal of tetracycline in synthetically concocted wastewater
The process of mechanically stirred membrane reactor containing the suspension of horseradish peroxidase (HRP) immobilized on synthesized...
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Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies
Spurred by advances in AI-driven modeling and experimental methods, molecular dynamics simulations are now acting as a platform to integrate these...
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Isolation and Characterization of Extracellular Vesicles from Lymphocytes
Extracellular vesicles (EVs) are nanosized particles secreted by all cells as a means of communication. When it comes to the immune system, most of...
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Modelling in Membrane Separation of Bioactives
Membrane based systems have become integral parts of the processing of bioactive compounds from the plant extract or fruit/vegetable juice. The...
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An Antimicrobial Peptide-Mimetic Methacrylate Random Copolymer Induces Domain Formation in a Model Bacterial Membrane
To address the emerging issue of drug-resistant bacteria, membrane-active synthetic polymers have been designed and developed to mimic host-defense...
