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Development of thermostable sucrose phosphorylase by semi-rational design for efficient biosynthesis of alpha-D-glucosylglycerol
AbstractSucrose phosphorylase (SPase) can specifically catalyze transglycosylation reactions and can be used to enzymatically synthesize...
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Semi-rational design of L-amino acid deaminase for production of pyruvate and d-alanine by Escherichia coli whole-cell biocatalyst
In our previous study, one-step pyruvate and d -alanine production from d , l -alanine by a whole-cell biocatalyst Escherichia coli expressing l -amino...
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Significantly improving the thermostability of a hyperthermophilic GH10 family xylanase XynAF1 by semi-rational design
AbstractXylanases have a broad range of applications in industrial biotechnologies, which require the enzymes to resist the high-temperature...
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Improving enzyme activity of glucosamine-6-phosphate synthase by semi-rational design strategy and computer analysis
ObjectiveTo improve enzyme activity of Glucosamine-6-phosphate synthase (Glms) of Bacillus subtilis by site saturation mutagenesis at Leu 593 , Ala 594 ,...
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Genome mining integrating semi-rational protein engineering and nanoreactor design: roadmap for a robust biocatalyst for industrial resolution of Vince lactam
Biomanufacturing of chemicals using biocatalysts is an attractive strategy for the production of valuable pharmaceuticals since it is usually more...
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Machine learning for functional protein design
Recent breakthroughs in AI coupled with the rapid accumulation of protein sequence and structure data have radically transformed computational...
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Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus
ObjectivesAlthough Geobacillus are significant thermophilic bacteria source, there are no reports of thermostable esterase gene in Geobacillus...
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Design and evaluation of tadpole-like conformational antimicrobial peptides
Antimicrobial peptides are promising alternatives to conventional antibiotics. Herein, we report a class of “tadpole-like” peptides consisting of an...
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Covalent-reversible peptide-based protease inhibitors. Design, synthesis, and clinical success stories
Dysregulated human peptidases are implicated in a large variety of diseases such as cancer, hypertension, and neurodegeneration. Viral proteases for...
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Sciomyzidae (Diptera) Assemblages in Constructed and Natural Wetlands: Implications for Constructed Wetland Design
Wetlands constructed primarily for the treatment of wastewaters have been shown to have a role in enhancing biodiversity. However, while most...
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Thermostability engineering of industrial enzymes through structure modification
AbstractThermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus,...
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A growth selection system for sucrose synthases (SuSy): design and test
High throughput screening (HTS) methods of enzyme variants are essential for the development of robust biocatalysts suited for low impact, industrial...
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Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short...
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Phenylalanine ammonia-lyases: combining protein engineering and natural diversity
AbstractIn this study, rational design and saturation mutagenesis efforts for engineering phenylalanine ammonia-lyase from Petroselinum crispum ( Pc PA...
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Current advances in design and engineering strategies of industrial enzymes
Enzymes are considered as functional proteins, also known as biocatalysts, which are required for normal cellular function. The commercial production...
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Reconciling East-African Wetland Conservation with Human Needs: Managing Uncertainties in Environmental Policy Design
Rapidly develo** societies in East-Africa impose increasing pressures on wetlands due to rising food demand and degradation of upland soils....
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Drug Design Methods to Regulate Protein–Protein Interactions
Inhibiting anomalous protein–protein interactions has led to the discovery of drugs (small molecules, precisely) that can inhibit such interactions...
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Designing a Model-Driven Approach Towards Rational Experimental Design in Bioprocess Optimization
To enable a more rational optimization approach to drive the transition from lab-scale to large industrial bioprocesses, a systematic framework...
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Semi-rational mutagenesis of an industrial Streptomyces fungicidicus strain for improved enduracidin productivity
The biosynthesis of the valuable antibiotic enduracidin by Streptomyces fungicidicus TXX3120 is a complex multistep process. To identify the...
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Design and Operation of New Microbial Product Bioprocessing System
Microbial products are recognized as the most diverse commercially important chemical structure. Bioprocess offers sustainable techniques for the...