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A recombinase polymerase amplification–SYBR Green I assay for the rapid and visual detection of Brucella
Brucellosis is a zoonosis caused by Brucella , which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important...
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Quantitative PCR of Alu Repeats Using PowerUp™ SYBR® Green Master Mix
Quantitative PCR is one of the fundamental steps performed when processing routine casework in a forensic laboratory. Quantitative PCR of Alu repeats...
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Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
AbstractThe pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For develo** countries where vaccines are still in...
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Comprehensive and species-specific detection of mackerels of the genus Scomber in processed foods using SYBR Green-based real-time PCR
The genus Scomber , commonly known as mackerel, is recognized as a potential allergenic fish species, necessitating the assessment of Scomber species...
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Preparation and Physical Characterization of DNA Binding Cationic Liposomes
Cationic liposomes are routinely employed as one of the major nonviral transfecting agents for intracellular delivery of hydrophilic molecules such...
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Advancing Nematode Identification on Potato: An Isothermal Recombinase Polymerase Amplification Assay for Stubby Root Nematode, Paratrichodorus allius
The stubby root nematode, Paratrichodorus allius , is an important plant-parasitic nematode that feeds on plant roots and transmits Tobacco rattle virus ...
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DUSP4 modulates RIG-I- and STING-mediated IRF3-type I IFN response
Detection of cytosolic nucleic acids by pattern recognition receptors, including STING and RIG-I, leads to the activation of multiple signalling...
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The response of LncRNAs associated with photosynthesis-and pigment synthesis-related genes to green light in Chlamydomonas reinhardtii
The quality of light is an important abiotic factor that affects the growth and development of green plants. Ultraviolet, red, blue, and far-red...
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Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo
Ultrasmall algae have attracted the attention of biologists investigating the basic mechanisms underlying living systems. Their potential as...
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qPCR Assay in sxtA Gene in Saxitoxin-Producing Cyanobacteria
Saxitoxin, as one type of paralytic shellfish toxin, can bind to sodium channels and modulate the flux of sodium in nerve and muscle cells....
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Transcriptomic profiles of the ruminal wall in Italian Mediterranean dairy buffaloes fed green forage
BackgroundGreen feed diet in ruminants exerts a beneficial effect on rumen metabolism and enhances the content of milk nutraceutical quality. At...
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qPCR Assay for the Detection of Hepatotoxigenic Cyanobacteria
Harmful algal blooms are contaminating fresh and marine waterbodies. The frequency and toxicity of these algal blooms are predicted to rise at an...
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Leafhopper transmits soybean stay-green associated virus to leguminous plants
A novel geminivirus, soybean stay-green associated virus (SoSGV), was previously shown to cause soybean delayed senescence and is associated with the...
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A large deletion conferring pale green leaves of maize
BackgroundThe structural basis of chloroplast and the regulation of chloroplast biogenesis remain largely unknown in maize. Gene mutations in these...
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Different characteristics of multidrug-resistant isolates of Pseudomonas aeruginosa in in vitro and in vivo conditions
Bacterial virulence factors play a key role in the outcome of burn wound infections. We aimed to assess the differences in virulence properties of...
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SARS-CoV-2 ORF6 protein targets TRIM25 for proteasomal degradation to diminish K63-linked RIG-I ubiquitination and type-I interferon induction
Evasion and antagonism of host cellular immunity upon SARS-CoV-2 infection provide replication advantage to the virus and contribute to COVID-19...
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Transcriptome analysis of hypothalamus and pituitary tissues reveals genetic mechanisms associated with high egg production rates in Changshun green-shell laying hens
BackgroundChangshun green-shell laying hens are unique to the Guizhou Province, China, and have high egg quality but relatively low yield. Egg...
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Fluorescent Techniques for RNA Detection in Nanoparticles
The utilization of drug delivery systems, such as lipid nanoparticles and polyplexes/micelleplexes, has shown promise in intracellularly delivering...
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In vitro Effects of Cold Shock on the Size and Activity of Nucleated Erythrocytes in Scorpaena porcus (Linnaeus, 1758)
AbstractThe in vitro effect of cold shock on nucleated erythrocytes of the benthic scorpionfish Scorpaena porcus L. was studied. Blood samples were...
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VRK1 promotes DNA-induced type I interferon production
BackgroundType I interferons (IFNs) are an essential class of cytokines with antitumor, antiviral and immunoregulatory effects. However,...
