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Protoplast Isolation and Transfection in Rice
Rice (Oryza sativa) is an important cereal crop and a model monocot plant for biology research. The reliable system of foreign DNA transformation and...
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Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target...
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Genome editing of RICE FLOWERING LOCUS T 1 promoter delayed flowering in rice
The heading date of rice is a crucial agronomic traits affecting regional adaptation and grain productivity. Manipulating the vegetative growth...
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Development of a Mesophyll Protoplast-Based System for Gene Editing of Papaya
Carica papaya L. is an economically significant crop in tropical and subtropical regions, with a gross production value of $6.2 × 10 9 in 2020....
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A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza
Key messageA high-efficiency protoplast transient system was devised to screen genome editing elements in Salvia miltiorrhiza .
Abstract ...
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An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast
BackgroundThe bimolecular fluorescence complementation (BiFC) assay is commonly used for investigating protein–protein interactions. While several...
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A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass
BackgroundWith the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts,...
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Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis
Protoplasts are a versatile and powerful cell-based system to study different plant processes in vivo, due to their ability to maintain cell identity...
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Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper ( Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily...
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Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples
The genus Brassica includes vegetables such as broccoli, cabbage, cauliflower, kale, and kohlrabi, which are economically important because they...
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Establishment of protoplast preparation protocol and genetic transformation system for Sclerotiophoma versabilis
Going into deep study about the molecular mechanism of pathogenesis in Sclerotiophoma versabilis required high-efficiency transformation system. S....
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Efficient mesophyll-derived protoplast manipulation system as a versatile tool for characterization of genes responding to multiple stimuli in mulberry
Mulberry serves as a crucial element of the global sericulture industry. Functional gene analysis considering resistant traits improvement of...
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Production and characterization of somatic hybrids between rice (Oryza sativa L.) and reed (Phragmites communis Trin.) obtained by protoplast fusion
Protoplasts were isolated from cell suspension cultures of rice ( Oryza sativa L.) and reed ( Phragmites communis Trin.), fused by a PEG–high pH-high Ca
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A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]
BackgroundTea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its...
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Efficient leaf mesophyll protoplast isolation and transient gene expression in bermudagrasses (Cynodon spp.)
Bermudagrasses ( Cynodon spp. ) are important warm-season turfgrass species with multiple applications. Although physiological and high-throughput...
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Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies
In an era of cost-efficient gene synthesis and high-throughput construct assembly, the onus of scientific experimentation is on the rate of in vivo...
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Physcomitrium patens Protoplasting and Protoplast Transfection
Protoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base...
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Highly efficient endosperm and pericarp protoplast preparation system for transient transformation of endosperm-related genes in wheat
Plant protoplasts constitute a versatile system for transient gene expression and have frequently been used in high-throughput to screen and identify...
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Establishment of a PEG-Assisted Protoplast Transfection System in Musa acuminata cv. Berangan (AAA) Using a CRISPR/Cas9 Ribonucleoprotein Complex
AbstractBanana is an important food crop worldwide. However, as banana production is largely affected by pests, diseases, and environmental stresses,...
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Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
BackgroundCotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered...
