Search
Search Results
-
Replication fork uncoupling causes nascent strand degradation and fork reversal
Genotoxins cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial for genome stability...
-
Genome-wide identification of replication fork stalling/pausing sites and the interplay between RNA Pol II transcription and DNA replication progression
BackgroundDNA replication progression can be affected by the presence of physical barriers like the RNA polymerases, leading to replication stress...
-
Dynamic de novo heterochromatin assembly and disassembly at replication forks ensures fork stability
Chromatin is dynamically reorganized when DNA replication forks are challenged. However, the process of epigenetic reorganization and its implication...
-
Genome-wide measurement of DNA replication fork directionality and quantification of DNA replication initiation and termination with Okazaki fragment sequencing
Studying the dynamics of genome replication in mammalian cells has been historically challenging. To reveal the location of replication initiation...
-
Monitoring and quantifying replication fork dynamics with high-throughput methods
Before each cell division, eukaryotic cells must replicate their chromosomes to ensure the accurate transmission of genetic information. Chromosome...
-
Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis
Processivity clamps tether DNA polymerases to DNA, allowing their access to the primer–template junction. In addition to DNA replication, DNA...
-
Stalled replication fork protection limits cGAS–STING and P-body-dependent innate immune signalling
Protection of stalled replication forks is crucial for cells to respond to replication stress and maintain genome stability. Genome instability and...
-
Control of DNA replication in vitro using a reversible replication barrier
A major obstacle to studying DNA replication is that it involves asynchronous and highly delocalized events. A reversible replication barrier...
-
FORK-seq: Single-Molecule Profiling of DNA Replication
Most genome replication map** methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore...
-
FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine
Fanconi anemia (FA) is a rare hereditary disorder caused by mutations in any one of the FANC genes. FA cells are mainly characterized by extreme...
-
Ki-67 is necessary during DNA replication for fork protection and genome stability
BackgroundThe proliferation antigen Ki-67 has been widely used in clinical settings for cancer staging for many years, but investigations on its...
-
DNA replication and replication stress response in the context of nuclear architecture
The DNA replication process needs to be coordinated with other DNA metabolism transactions and must eventually extend to the full genome, regardless...
-
Replication stress as a driver of cellular senescence and aging
Replication stress refers to slowing or stalling of replication fork progression during DNA synthesis that disrupts faithful copying of the genome....
-
Crucial role of the NSE1 RING domain in Smc5/6 stability and FANCM-independent fork progression
The Smc5/6 complex is a highly conserved molecular machine involved in the maintenance of genome integrity. While its functions largely depend on...
-
The TIMELESS effort for timely DNA replication and protection
Accurate replication of the genome is fundamental to cellular survival and tumor prevention. The DNA replication fork is vulnerable to DNA lesions...
-
Essential role of CK2α for the interaction and stability of replication fork factors during DNA synthesis and activation of the S-phase checkpoint
The ataxia telangiectasia mutated and Rad3-related (ATR)-CHK1 pathway is the major signalling cascade activated in response to DNA replication...
-
Loo** out of control: R-loops in transcription-replication conflict
Transcription-replication conflict is a major cause of replication stress that arises when replication forks collide with the transcription...
-
Direct visualization of transcription-replication conflicts reveals post-replicative DNA:RNA hybrids
Transcription-replication collisions (TRCs) are crucial determinants of genome instability. R-loops were linked to head-on TRCs and proposed to...
-
Cytidine deaminases APOBEC3C and APOBEC3D promote DNA replication stress resistance in pancreatic cancer cells
Gemcitabine is a potent inhibitor of DNA replication and is a mainstay therapeutic for diverse cancers, particularly pancreatic ductal adenocarcinoma...
-
PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment
Telomeres are nucleoprotein structures at eukaryotic chromosome termini. Their stability is preserved by a six-protein complex named shelterin. Among...
