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RNA Transcription
The process of producing proteins from nucleotides is termed as gene expression. Genetic information present in the DNA, is first rewritten,... -
Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions
RNA performs a wide variety of vital cellular functions. These functions typically require interactions with other biological macromolecules, often... -
Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription
Large-scale and cost-less production of double-stranded RNA (dsRNA) is the basis for the widespread application of dsRNA in agriculture.... -
Bi-directional Transcription, Double-Stranded RNA and the Implications on Genome Evolution
Double-stranded RNA (dsRNA) within cells indicates viral infection and triggers a robust innate immune response. Counterintuitively, cells also... -
Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology
Abstract —RNA polymerase III synthesizes a wide range of noncoding RNAs shorter than 400 nucleotides in length. These RNAs are involved in protein...
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The regulatory relationship between transcription factor STAT3 and noncoding RNA
Signal transducer and activator of transcription 3 (STAT3), as a key node in numerous carcinogenic signaling pathways, is activated in various tumor...
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Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing
Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We...
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LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis
Existing single-cell RNA sequencing (scRNA-seq) methods rely on reverse transcription (RT) and second-strand synthesis (SSS) to convert...
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Transfer RNA modifications and DNA editing in HIV-1 reverse transcription
Reverse transcription is a central step in HIV-1 replication that represents a typical case of interplay between viral and cellular factors. HIV-1... -
Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair
During transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that...
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Nuclear Transcription Factor Detection
Cellular fate is determined by the activity of nuclear transcription factors. Here, we describe a series of protocols for detecting transcription... -
Intra-promoter switch of transcription initiation sites in proliferation signaling-dependent RNA metabolism
Global changes in transcriptional regulation and RNA metabolism are crucial features of cancer development. However, little is known about the role...
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Profiling of RNA-binding protein binding sites by in situ reverse transcription-based sequencing
RNA-binding proteins (RBPs) regulate diverse cellular processes by dynamically interacting with RNA targets. However, effective methods to capture...
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Regulation of ribosomal RNA gene copy number, transcription and nucleolus organization in eukaryotes
One of the first biological machineries to be created seems to have been the ribosome. Since then, organisms have dedicated great efforts to optimize...
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Genome-wide identification of replication fork stalling/pausing sites and the interplay between RNA Pol II transcription and DNA replication progression
BackgroundDNA replication progression can be affected by the presence of physical barriers like the RNA polymerases, leading to replication stress...
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Circular RNA circSP5 promotes liver metastasis of colorectal cancer via SP5-mediated BAMBI transcription
Liver metastasis of colorectal cancer (CRC) is a major cause of cancer morbidity and mortality. Circular RNAs (circRNAs) have been widely reported to...
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Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II
RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes...
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Genomic regulation of transcription and RNA processing by the multitasking Integrator complex
In higher eukaryotes, fine-tuned activation of protein-coding genes and many non-coding RNAs pivots around the regulated activity of RNA polymerase...
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Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases
The eukaryotic transcription apparatus synthesizes a staggering diversity of RNA molecules. The labour of nuclear gene transcription is, therefore,...