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Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology
BackgroundGenomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are...
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Genetic Transformation System
Lingzhi (Ganoderma lucidum) contains various bioactive constituents, such as polysaccharides and triterpenoids. The development of genetic...
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Efficient Agrobacterium tumefaciens-mediated genetic transformation of Aloe vera
Genetic transformation of plants has emerged as a core research tool for the functional characterization of genes and cultivar improvement in the...
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Agrobacterium tumefaciens-Mediated Transformation Method for Fusarium oxysporum
Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming a popular effective system as an insertional mutagenesis tool in filamentous...
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High efficiency transformation and mutant screening of the laurel wilt pathogen, Raffaelea lauricola
AbstractThe fungal pathogen, Raffaelea lauricola , is the causative agent of laurel wilt, a devastating disease affecting the Lauraceae family. The...
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Influence of various cooking treatments and extraction solvents on bioactive compounds and antioxidant capacities of bottle gourd (Lagenaria siceraria) fruit in India
Bottle gourd is a good source of health-beneficial bioactive substances. This research aimed at evaluating the effect of different cooking methods...
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Genetic Transformation of Filamentous Fungi: Achievements and Challenges
Genetic transformation methods are a prerequisite for the functional analysis and modification of genes. About 30 years ago, first reports on the...
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A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii
Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving...
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A novel gene, Le-Dd10, is involved in fruiting body formation of Lentinula edodes
The cDNA library prepared from Lentinula edodes , Hokken 600 (H600), primordia was screened using cDNA expressed specifically in Dictyostelium...
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Exogenous polyamines enhance somatic embryogenesis and Agrobacterium tumefaciens-mediated transformation efficiency in sugarcane (Saccharum spp. hybrid)
The influence of exogenous polyamines (PAs) on somatic embryogenesis from immature leaf roll explants and Agrobacterium tumefaciens -mediated...
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Interplay of hormones and metabolite excretion with fern pattern prove saliva as a potent indicator of male reproductive status in Kangayam breed cattle
Kangayam cattle are one of the drought breeds in India with distinct attributes. Agricultural transformation has led to a decline in many pure-breed...
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Transgenic rice expressing Trichoderma spp. endochitinase gene exhibits moderate resistance to sheath blight
Rice is a staple food consumed by almost half of the world’s population. The crop is prone to a myriad of diseases, and sheath blight caused by Rhizoct...
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Molecular phylogenetic identification of Metarhizium and Beauveria and their bio-efficacy against chilli thrips, Scirtothrips dorsalis
Molecular phylogenetic species recognition is essential in precisely differentiating the morphologically cryptic species of Metarhizium and Beauveria. ...
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Kinetics and Dynamics of Conversion of Bambusa Bamboo into 5-Hydroxymethylfurfural in Ionic Solvent in Batch Reactor
In this era of industrial revolution and diminishing petroleum reserves, alternative options (woody biomass) for building next-generation fuels need...
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Phaseolus vulgaris-Colletotrichum lindemuthianum Pathosystem in the Post-Genomic Era: An Update
Phaseolus vulgaris—Colletotrichum lindemuthianum is one among the oldest host and pathogen interface. Researchers have taken painstaking efforts...
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Endophytic Fungi: Potential Source of Allelochemicals for Sustainable Agriculture
A diverse group of microorganisms known as plant-associated fungi, also known as endophytic fungi, are usually found anonymously between the cell’s...
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Restriction enzyme-mediated insertional mutagenesis: an efficient method of Rosellinia necatrix transformation
Rosellinia necatrix : causing root rot disease is a very destructive pathogen of woody plants and is responsible for yield losses to a large number of...
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Range-wide and temporal genomic analyses reveal the consequences of near-extinction in Swedish moose
Ungulate species have experienced severe declines over the past centuries through overharvesting and habitat loss. Even if many game species have...
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High nuclear expression of DNMT1 in correlation with inactivation of TET1 portray worst prognosis among the cervical carcinoma patients: clinical implications
In this study, we aimed to understand the interplay of the epigenetic modifier genes DNMT1 and TET1 along with HPV infection in the cervical...
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Heparin coated decellularized xenogeneic small diameter vascular conduit for vascular repair with early luminal reendothelialization
Small diameter vascular graft is a clinical need in cardiovascular disease (CAD) and peripheral atherosclerotic diseases (PAD). Autologous graft has...
