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  1. Enhanced recombination empowers the detection and map** of Quantitative Trait Loci

    Modern plant breeding, such as genomic selection and gene editing, is based on the knowledge of the genetic architecture of desired traits....

    Laia Capilla-Pérez, Victor Solier, ... Raphael Mercier in Communications Biology
    Article Open access 08 July 2024
  2. Rapid and quantitative detection of Aspergillus niger Van Tieghem using loop-mediated isothermal amplification assay

    Peanut ( Arachis hypogaea L.) crown rot and root rot are common diseases caused by Aspergillus niger Van Tieghem. Early and accurate detection of A....

    **aodong Dai, Yanyong Cao, ... **nyou Zhang in Journal of Plant Pathology
    Article Open access 25 June 2024
  3. Quantitative detection of chikungunya, Zika, and dengue viruses by one-step real-time PCR in different cell substrates

    Chikungunya (CHIKV), Zika (ZIKV), and dengue viruses (DENV) are vector-borne pathogens that cause emerging and re-emerging epidemics throughout...

    Andrés F. Cuellar-Quimbaya, Ana Luisa Muñoz, ... Mónica Losada-Barragán in Brazilian Journal of Microbiology
    Article 01 March 2024
  4. Quantitative Peptidomics: General Considerations

    Peptidomics is the detection and identification of the peptides present in a sample, and quantitative peptidomics provides additional information...
    Lloyd D. Fricker in Peptidomics
    Protocol 2024
  5. Statistical and Quantitative Genetics Studies

    Quantitative genetics and plant breeding are non-exclusive and these disciplines has been benefited from each other for the past 100 years. As a...
    Rumesh Ranjan, Wajhat Un Nisa, ... Yogesh Vikal in Genomics Data Analysis for Crop Improvement
    Protocol 2024
  6. Identification of s9ap used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of Pleurotus eryngii

    Background

    Pleurotus eryngii is a kind of edible fungi with good quality, and it is popular among consumers. At present, some adulterated edible...

    Yuanmiao Wei, Yao Liu, ... Ying Shang in Molecular Biology Reports
    Article 12 November 2022
  7. Quantitative detection of Vibrio alginolyticus strain XSBZ14 by a newly developed RT-PCR method

    Purpose

    Coral degradation is a worldwide ecological problem. Bacterial diseases are a great danger to coral health. The pathogenic bacterium Vibrio...

    Na Zhang, Siyue Yang, ... Zhenyu **e in Annals of Microbiology
    Article Open access 19 August 2023
  8. Development of a quantitative PCR assay for the detection of Piggotia coryli, the causal agent of hazelnut anthracnose

    A novel real-time PCR assay was developed for the detection of Piggotia coryli, the causal agent of hazelnut anthracnose. A molecular tool for...

    Mounira Inas Drais, Silvia Turco, ... Angelo Mazzaglia in Journal of Plant Pathology
    Article 13 February 2023
  9. Quantitative multiplex real-time polymerase chain reaction assay for the detection of Helicobacter pylori and clarithromycin resistance

    Background

    Identifying clarithromycin resistance is essential for eradicating Helicobacter pylori (HP). Therefore, we evaluated the performance of...

    Ilsoo Kim, Lee-So Maeng, ... Soo-heon Park in BMC Microbiology
    Article Open access 27 May 2023
  10. Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant

    Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia...

    Cleudison Gabriel Nascimento Da Silva, Edevaldo de Castro Monteiro, ... Segundo Urquiaga in Brazilian Journal of Microbiology
    Article 16 October 2023
  11. Linkage map** and quantitative trait loci detection for seedling vigor and grain size in advanced backcross introgression lines from wild accessions of Oryza nivara

    Detection of beneficial genes from wild accessions of cultivated crops is becoming increasingly important for crop improvement, especially for traits...

    P. Magudeeswari, Divya Balakrishnan, ... N. Sarla in Genetic Resources and Crop Evolution
    Article 06 January 2024
  12. Quantitative Biochemical Analysis of Deadenylase Enzymes Using Fluorescence and Chemiluminescence-Based Assays

    Deadenylase enzymes play a key role in mRNA degradation and RNA processing. In this chapter, we describe two activity assays for the quantitative...
    Blessing Airhihen, Lorenzo Pavanello, ... Gerlof Sebastiaan Winkler in Deadenylation
    Protocol 2024
  13. Quantitative trait loci detection for three tiller-related traits and the effects on wheat (Triticum aestivum L.) yields

    Key message

    A total of 38 putative additive QTLs and 55 pairwise putative epistatic QTLs for tiller-related traits were reported, and the candidate...

    Yibiao Cai, **aohan Zhou, ... Fa Cui in Theoretical and Applied Genetics
    Article 21 March 2024
  14. Algorithms for Quantitative Pedology

    The Algorithms for Quantitative Pedology (AQP) project consists of a suite of packages for the R programming language that simplify quantitative...
    D. E. Beaudette, J. Skovlin, ... S. M. Roecker in Geopedology
    Chapter 2023
  15. Immunoassay for Quantitative Detection of Antibody Transcytosis Across the Blood-Brain Barrier In Vitro

    Automated high-throughput immunoassays are emerging as reliable analytic techniques for the quantitative detection of proteins from a variety of...
    Caroline Sodja, Deborah Callaghan, ... Anna Jezierski in Induced Pluripotent Stem Cells and Human Disease
    Protocol 2022
  16. Design and Validation of Primer Sets for the Detection and Quantification of Antibiotic Resistance Genes in Environmental Samples by Quantitative PCR

    The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Particularly,...

    Lizandra Perez-Bou, Alejandro Gonzalez-Martinez, ... David Correa-Galeote in Microbial Ecology
    Article Open access 15 May 2024
  17. Development of a multiplex quantitative PCR assay for simultaneous detection of Treponema phagedenis, Treponema pedis, Treponema medium, and ‘Treponema vincentii’ and evaluation on bovine digital dermatitis biopsies

    Bovine digital dermatitis (BDD) is a contagious foot disease with worldwide occurrence in dairy cattle. The disease causes lameness and reduced...

    Sara Frosth, Hanna K. Eriksson, Anna Rosander in Veterinary Research Communications
    Article Open access 01 June 2023
  18. Rapid assessment of invasion front and biological impact of the invasive fish Coreoperca herzi using quantitative eDNA metabarcoding

    Early detection and rapid response are crucial for minimizing the impacts of biological invasions on biodiversity, ecosystem functioning, and...

    Satsuki Tsuji, Hideyuki Doi, ... Katsutoshi Watanabe in Biological Invasions
    Article 12 June 2024
  19. Detecting fossorial salamanders using eDNA: Development and validation of quantitative and end-point PCR assays for the detection of five species of Ambystoma

    In the past decade, quantification of environmental DNA (eDNA) has become firmly established as an effective method for detecting the presence of...

    Ben F. Brammell, Elizabeth K. Strasko, ... Malinda A. Stull in Conservation Genetics Resources
    Article 04 October 2023
  20. Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR

    Assessment of DNA base and strand damage can be determined using a quantitative PCR assay that is based on the concept that damage blocks the...
    Lang Pan, Yaoyao Xue, ... Istvan Boldogh in Base Excision Repair Pathway
    Protocol 2023
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