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Enhanced recombination empowers the detection and map** of Quantitative Trait Loci
Modern plant breeding, such as genomic selection and gene editing, is based on the knowledge of the genetic architecture of desired traits....
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Rapid and quantitative detection of Aspergillus niger Van Tieghem using loop-mediated isothermal amplification assay
Peanut ( Arachis hypogaea L.) crown rot and root rot are common diseases caused by Aspergillus niger Van Tieghem. Early and accurate detection of A....
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Quantitative detection of chikungunya, Zika, and dengue viruses by one-step real-time PCR in different cell substrates
Chikungunya (CHIKV), Zika (ZIKV), and dengue viruses (DENV) are vector-borne pathogens that cause emerging and re-emerging epidemics throughout...
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Quantitative Peptidomics: General Considerations
Peptidomics is the detection and identification of the peptides present in a sample, and quantitative peptidomics provides additional information... -
Statistical and Quantitative Genetics Studies
Quantitative genetics and plant breeding are non-exclusive and these disciplines has been benefited from each other for the past 100 years. As a... -
Identification of s9ap used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of Pleurotus eryngii
BackgroundPleurotus eryngii is a kind of edible fungi with good quality, and it is popular among consumers. At present, some adulterated edible...
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Quantitative detection of Vibrio alginolyticus strain XSBZ14 by a newly developed RT-PCR method
PurposeCoral degradation is a worldwide ecological problem. Bacterial diseases are a great danger to coral health. The pathogenic bacterium Vibrio...
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Development of a quantitative PCR assay for the detection of Piggotia coryli, the causal agent of hazelnut anthracnose
A novel real-time PCR assay was developed for the detection of Piggotia coryli, the causal agent of hazelnut anthracnose. A molecular tool for...
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Quantitative multiplex real-time polymerase chain reaction assay for the detection of Helicobacter pylori and clarithromycin resistance
BackgroundIdentifying clarithromycin resistance is essential for eradicating Helicobacter pylori (HP). Therefore, we evaluated the performance of...
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Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant
Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia...
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Linkage map** and quantitative trait loci detection for seedling vigor and grain size in advanced backcross introgression lines from wild accessions of Oryza nivara
Detection of beneficial genes from wild accessions of cultivated crops is becoming increasingly important for crop improvement, especially for traits...
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Quantitative Biochemical Analysis of Deadenylase Enzymes Using Fluorescence and Chemiluminescence-Based Assays
Deadenylase enzymes play a key role in mRNA degradation and RNA processing. In this chapter, we describe two activity assays for the quantitative... -
Quantitative trait loci detection for three tiller-related traits and the effects on wheat (Triticum aestivum L.) yields
Key messageA total of 38 putative additive QTLs and 55 pairwise putative epistatic QTLs for tiller-related traits were reported, and the candidate...
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Algorithms for Quantitative Pedology
The Algorithms for Quantitative Pedology (AQP) project consists of a suite of packages for the R programming language that simplify quantitative... -
Immunoassay for Quantitative Detection of Antibody Transcytosis Across the Blood-Brain Barrier In Vitro
Automated high-throughput immunoassays are emerging as reliable analytic techniques for the quantitative detection of proteins from a variety of... -
Design and Validation of Primer Sets for the Detection and Quantification of Antibiotic Resistance Genes in Environmental Samples by Quantitative PCR
The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Particularly,...
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Development of a multiplex quantitative PCR assay for simultaneous detection of Treponema phagedenis, Treponema pedis, Treponema medium, and ‘Treponema vincentii’ and evaluation on bovine digital dermatitis biopsies
Bovine digital dermatitis (BDD) is a contagious foot disease with worldwide occurrence in dairy cattle. The disease causes lameness and reduced...
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Rapid assessment of invasion front and biological impact of the invasive fish Coreoperca herzi using quantitative eDNA metabarcoding
Early detection and rapid response are crucial for minimizing the impacts of biological invasions on biodiversity, ecosystem functioning, and...
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Detecting fossorial salamanders using eDNA: Development and validation of quantitative and end-point PCR assays for the detection of five species of Ambystoma
In the past decade, quantification of environmental DNA (eDNA) has become firmly established as an effective method for detecting the presence of...
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Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR
Assessment of DNA base and strand damage can be determined using a quantitative PCR assay that is based on the concept that damage blocks the...