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Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia
BackgroundSince the discovery more than half a century ago, cell-free DNA (cfDNA) has become an attractive objective in multiple diagnostic,...
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Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection
Viral infection disrupts the normal regulation of the host gene’s expression. In order to normalise the expression of dysregulated host genes upon...
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Evaluation of reference genes for qRT-PCR studies in the colchicine producing Gloriosa superba L.
The flame lily , Gloriosa superba L., is one of the two primary sources of the anti-inflammatory drug, colchicine. Previous studies have shown that a...
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Identification and testing of reference genes for qRT-PCR analysis during pear fruit development
Quantitative real-time PCR (qRT-PCR) is currently one of the most reliable and improved tools for analyzing gene expression. Various studies have...
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qRT-PCR Analysis of GLUT-4 and Assessment of Trolox as an Effective Antioxidant in Diabetic Cardiomyoblasts
A high global prevalence of diabetes and its implications on the heart in vivo and in vitro tools have been pursued to alleviate the complications of...
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Assessment of thermal and temporal stability of SARS-CoV-2 samples using real-time qRT-PCR
BackgroundAs per the guidelines of the Indian Council of Medical Research, nasopharyngeal and oropharyngeal swabs in viral transport medium (VTM) are...
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Stem-Loop qRT-PCR–Based Quantification of miRNAs
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification...
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Identification and validation of reference genes for qRT-PCR based studies in horse gram (Macrotyloma uniflorum)
The quantitative real-time polymerase chain reaction (qRT-PCR) is the most sensitive and commonly used technique for gene expression studies in...
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Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
BackgroundCoronavirus disease of 2019 (COVID-19) is well known as a fatal disease, first discovered at Wuhan in China, ranging from mild to death,...
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Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in Rice
With a widely established use of quantitative real-time PCR (qRT-PCR) for gene expression analysis, reliable and stable expression of reference genes...
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Identification of suitable reference genes for expression profiling studies using qRT-PCR in an important insect pest, Maruca vitrata
BackgroundMaruca vitrata is one of the potential insect pests that cause devastating losses to legume cultivation worldwide. Gene functional studies...
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Characterisation and validation of housekee** genes for qRT-PCR expression analysis in Pterophyllum scalare
Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive and advanced method to quantify the expression of target genes of animals including...
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Evaluation of suitable qRT-PCR normalization genes for various citrus rootstocks
Citrus rootstock can modify plant growth and enhance stress resistance. There are many genotypes and species used as citrus rootstocks. Although...
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RETRACTED ARTICLE: Detection of HPV16 viral load in L2 gene as a related predictor of cervical cancer among women in Dhi-Qar province by qRT-PCR
BackgroundThe most common infection among young women that increases the risk of develo** cervical cancer (CC) is human papillomavirus (HPV). In...
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Stepwise Optimization of Real-Time RT-PCR Analysis
Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the...
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Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues
Real-time, or quantitative, reverse transcription polymerase chain reaction (qRT-PCR) is a powerful method for rapid and reliable quantification of...
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Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions
Scutellaria baicalensis Georgi is a famous medicinal plant with its dried roots having been used as a traditional Chinese medicinal for more than...
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An Enhanced Quantitative Reverse Transcription-PCR Approach for Measuring Circulating MicroRNAs
Short (22 nt) RNAs called microRNAs (miRNAs) bind to and inhibit target messenger RNAs in gene regulatory networks. Recent study suggests that miRNAs...
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Salinity stress reveals three types of RNA editing sites in mitochondrial Nad7 gene of wild barley both in silico and in qRT-PCR experiments
Cellular respiration is an important process performed by mitochondria. Nad complex is the major complex involved in this process and one of the main...
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Determination of Differential Alternative Splicing Under Stress Conditions
Alternative splicing (AS) is an important mechanism contributing to stress-induced regulation of gene expression and proteome diversity. Massive...