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Establishment of protoplast preparation protocol and genetic transformation system for Sclerotiophoma versabilis
Going into deep study about the molecular mechanism of pathogenesis in Sclerotiophoma versabilis required high-efficiency transformation system. S....
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Viable protoplast isolation, organelle visualization and transformation of the globally distributed plant pathogen Phytophthora cinnamomi
Phytophthora cinnamomi is an oomycete plant pathogen with a host range of almost 5000 plant species worldwide and therefore poses a serious threat to...
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Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)
BackgroundAreca palm ( Areca catechu ) is a woody perennial plant of both economical and medicinal importance grown in tropical and subtropical...
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Protonema induction, transient transformation, and protoplast regeneration in the peat moss Sphagnum papillosum
Sphagnum mosses are important carbon sequesters and emerging model organisms. However, induction and long-term cultivation of thalloid protonema in...
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Highly efficient endosperm and pericarp protoplast preparation system for transient transformation of endosperm-related genes in wheat
Plant protoplasts constitute a versatile system for transient gene expression and have frequently been used in high-throughput to screen and identify...
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Development of lead (Pb) tolerant strain by protoplast technology and their remediation
The lead is poisonous metal and because of its chemical nature it acts as an environmental contaminant through the water or soil and it becomes toxic...
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Establishment and optimization of PEG-mediated protoplast transformation in the microalga Haematococcus pluvialis
Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble...
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Efficient mesophyll-derived protoplast manipulation system as a versatile tool for characterization of genes responding to multiple stimuli in mulberry
Mulberry serves as a crucial element of the global sericulture industry. Functional gene analysis considering resistant traits improvement of...
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Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation
Key messageWe have developed and validated an efficient protocol for producing gene-edited carrot plants that do not result in the stable...
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Protoplast Isolation and Transformation in Oil Palm
The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol...
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A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza
Key messageA high-efficiency protoplast transient system was devised to screen genome editing elements in Salvia miltiorrhiza .
Abstract ...
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A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum
Red rot of sugarcane caused by the hemi-biotrophic fungal pathogen, Colletotrichum falcatum , is a major threat to sugarcane cultivation in many...
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Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper ( Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily...
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Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target...
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A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass
BackgroundWith the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts,...
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Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses
Forage and turf grasses are widely grown and contribute significantly to sustainable agriculture. This chapter describes a protocol for protoplast...
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Establishment of a PEG-Assisted Protoplast Transfection System in Musa acuminata cv. Berangan (AAA) Using a CRISPR/Cas9 Ribonucleoprotein Complex
AbstractBanana is an important food crop worldwide. However, as banana production is largely affected by pests, diseases, and environmental stresses,...
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Development of a Mesophyll Protoplast-Based System for Gene Editing of Papaya
Carica papaya L. is an economically significant crop in tropical and subtropical regions, with a gross production value of $6.2 × 10 9 in 2020....
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Isolation, culture of Platycodon grandiflorus protoplasts: factors affecting protoplast yield, cell division, and micro-callus formation
Plyatocodon grandiflorus is an important medicinal herb, its rhizomes are utilized as vegetables and as medicinal food. In this investigation, we...
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Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn
BackgroundFagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds....
