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Protoplast Technology Methods and Protocols
This detailed volume collects protocols from scientists who are actively engaged in develo** or using protoplast technology. The book begins with...
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Development of lead (Pb) tolerant strain by protoplast technology and their remediation
The lead is poisonous metal and because of its chemical nature it acts as an environmental contaminant through the water or soil and it becomes toxic...
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Phytosulfokine promotes cell division in protoplast culture and adventitious shoot formation in protoplast-derived calluses of Nicotiana benthamiana
Phytosulfokines (PSK) belong to a new class of peptide growth factors involved in intercellular signaling in plants. In this study, we examined the...
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Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples
The genus Brassica includes vegetables such as broccoli, cabbage, cauliflower, kale, and kohlrabi, which are economically important because they...
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Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn
BackgroundFagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds....
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Protoplast Isolation for Plant Single-Cell RNA-seq
The growth and development of plants depends on diversified gene expression in different cell types. Compared to traditional bulk RNA sequencing,...
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Isolation, culture of Platycodon grandiflorus protoplasts: factors affecting protoplast yield, cell division, and micro-callus formation
Plyatocodon grandiflorus is an important medicinal herb, its rhizomes are utilized as vegetables and as medicinal food. In this investigation, we...
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Development of a Mesophyll Protoplast-Based System for Gene Editing of Papaya
Carica papaya L. is an economically significant crop in tropical and subtropical regions, with a gross production value of $6.2 × 10 9 in 2020....
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Protoplast isolation and transient gene expression in different petunia cultivars
The protocol optimized for Petunia hybrida cv. Mirage Rose produced high protoplast yields in 3 out of other 11 cultivars (Damask White, Dreams...
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Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper ( Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily...
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Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai
BackgroundAngelica Gigas (Purple parsnip) is an important medicinal plant that is cultivated and utilized in Korea, Japan, and China. It contains...
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A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza
Key messageA high-efficiency protoplast transient system was devised to screen genome editing elements in Salvia miltiorrhiza .
Abstract ...
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Establishment of protoplast preparation protocol and genetic transformation system for Sclerotiophoma versabilis
Going into deep study about the molecular mechanism of pathogenesis in Sclerotiophoma versabilis required high-efficiency transformation system. S....
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Efficient mesophyll-derived protoplast manipulation system as a versatile tool for characterization of genes responding to multiple stimuli in mulberry
Mulberry serves as a crucial element of the global sericulture industry. Functional gene analysis considering resistant traits improvement of...
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A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]
BackgroundTea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its...
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Efficient leaf mesophyll protoplast isolation and transient gene expression in bermudagrasses (Cynodon spp.)
Bermudagrasses ( Cynodon spp. ) are important warm-season turfgrass species with multiple applications. Although physiological and high-throughput...
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Establishment of efficient Cannabis (Cannabis sativa L.) protoplast isolation and transient expression condition
There has been a significant increase in interest in Cannabis ( Cannabis sativa L.) plants for the production of secondary metabolites (i.e.,...
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Using protoplast fusion to improve biocontrol ability of Fusarium oxysporum against Egyptian broomrapes (Phelipanche Aegyptiaca)
To effectively combat parasitic weeds, it is essential to develop advanced biocontrol agents capable of thriving in diverse environmental conditions....
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Viable protoplast isolation, organelle visualization and transformation of the globally distributed plant pathogen Phytophthora cinnamomi
Phytophthora cinnamomi is an oomycete plant pathogen with a host range of almost 5000 plant species worldwide and therefore poses a serious threat to...
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Embryogenic callus induction, proliferation, protoplast isolation, and PEG induced fusion in Camellia oleifera
Camellia oleifera ( C. oleifera ) is one of the most important economic oil species in China. This study established an efficient protocol for the...
