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Phytosulfokine promotes cell division in protoplast culture and adventitious shoot formation in protoplast-derived calluses of Nicotiana benthamiana
Phytosulfokines (PSK) belong to a new class of peptide growth factors involved in intercellular signaling in plants. In this study, we examined the...
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Isolation, culture of Platycodon grandiflorus protoplasts: factors affecting protoplast yield, cell division, and micro-callus formation
Plyatocodon grandiflorus is an important medicinal herb, its rhizomes are utilized as vegetables and as medicinal food. In this investigation, we...
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Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat
Techniques based on the use of plant protoplasts are a convenient model for better understanding and observing developmental changes in the cells....
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Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai
BackgroundAngelica Gigas (Purple parsnip) is an important medicinal plant that is cultivated and utilized in Korea, Japan, and China. It contains...
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A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]
BackgroundTea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its...
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Development of lead (Pb) tolerant strain by protoplast technology and their remediation
The lead is poisonous metal and because of its chemical nature it acts as an environmental contaminant through the water or soil and it becomes toxic...
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Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn
BackgroundFagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds....
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Influences of factors affecting the induction of high protoplast yield and callus induction in two different carnations
This study aims to establish an efficient protocol for protoplast isolation, cell division, and callus induction in two carnation cultivars, Chabaud...
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Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples
The genus Brassica includes vegetables such as broccoli, cabbage, cauliflower, kale, and kohlrabi, which are economically important because they...
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Reconstruction pattern of the cell wall in Fagopyrum protoplast-derived hybrid cells
The cell wall rebuilding is one of the first stage of protoplast development that enables further mitotic divisions and differentiation. Therefore,...
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Embryogenic callus induction, proliferation, protoplast isolation, and PEG induced fusion in Camellia oleifera
Camellia oleifera ( C. oleifera ) is one of the most important economic oil species in China. This study established an efficient protocol for the...
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Efficient and rapid system of plant regeneration via protoplast cultures of Fagopyrum esculentum Moench
In the present study, a high yield of isolated protoplasts from the agronomically important crop Fagopyrum esculentum was obtained by applying a...
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A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza
Key messageA high-efficiency protoplast transient system was devised to screen genome editing elements in Salvia miltiorrhiza .
Abstract ...
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Efficient mesophyll-derived protoplast manipulation system as a versatile tool for characterization of genes responding to multiple stimuli in mulberry
Mulberry serves as a crucial element of the global sericulture industry. Functional gene analysis considering resistant traits improvement of...
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Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target...
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Establishment of protoplast preparation protocol and genetic transformation system for Sclerotiophoma versabilis
Going into deep study about the molecular mechanism of pathogenesis in Sclerotiophoma versabilis required high-efficiency transformation system. S....
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Protoplast for Gene Functional Analysis in Arabidopsis
Protoplast, a plant cell without cell wall, can be readily transfected by exogenous macromolecules (DNA, RNA, protein) and therefore offer a...
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Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)
Pepper ( Capsicum annuum L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily...
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Physcomitrium patens Protoplasting and Protoplast Transfection
Protoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base...
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Isolation, culture of protoplasts of Angelica gigas Nakai and regeneration of plants via somatic embryogenesis
In plant biotechnology, protoplasts are a versatile tool since they are very helpful for both fundamental biology studies and for genetic improvement...
