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Aniline blue test for sperm protamination—a valuable add-on to the bull breeding soundness evaluation
Bull breeding soundness evaluation (BBSE) is the most common procedure used to predict bull potential fertility. However, the use of traditional...
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CDKN2AIP is critical for spermiogenesis and germ cell development
BackgroundAs a member of RNA-binding protein, CDKN2AIP has been shown to play a critical role in stem cell pluripotency and somatic differentiation....
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Diversity of chromatin condensation patterns, nuclear reorganization, evolution and phylogenetic distribution of sperm nuclear basic proteins in fish
One of the most astonishing examples of chromatin remodelling occurs during the maturation of male germ cells, where changes in protein structure, as...
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Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein
Sperm chromatin compaction is physiologically essential for sperm to acquire the fertility. However, this unique structure composed of protamines...
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Cellular fate of intersex differentiation
Infertile ovotestis (mixture of ovary and testis) often occurs in intersex individuals under certain pathological and physiological conditions....
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Rapid evolutionary dynamics of an expanding family of meiotic drive factors and their hpRNA suppressors
Meiotic drivers are a class of selfish genetic elements whose existence is frequently hidden due to concomitant suppressor systems. Accordingly, we...
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Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids
BackgroundH1T2/H1FNT is a germ cell-specific linker histone variant expressed during spermiogenesis specifically in round and elongating spermatids....
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Casting histone variants during mammalian reproduction
During mammalian reproduction, germ cell chromatin packaging is key to prepare parental genomes for fertilization and to initiate embryonic...
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Entropy based analysis of vertebrate sperm protamines sequences: evidence of potential dityrosine and cysteine-tyrosine cross-linking in sperm protamines
BackgroundSpermatogenesis is the process by which germ cells develop into spermatozoa in the testis. Sperm protamines are small, arginine-rich...
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Evaluation of pliable bioresorbable, elastomeric aortic valve prostheses in sheep during 12 months post implantation
Pliable microfibrous, bioresorbable elastomeric heart valve prostheses are investigated in search of sustainable heart valve replacement. These...
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Recellularization of testicular feminization testis in C57bl6 as a natural bioreactor for creation of cellularized seminiferous tubules: an experimental study
We determined histological aspects of implanted human decellularized testicular matrix (DTM) in C57BL6 as a primitive step for further testis tissue...
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Echocardiographic Imaging of Cardiac Valves
Perioperative echocardiography (echo) has become the imaging modality of choice during cardiac valve surgery. It is a very useful tool not only for...
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Current Status of Therapeutic Peptides for the Management of Diabetes Mellitus
Diabetes mellitus (DM) is a chronic endocrine disorder with lifelong implications. The prevalence of this condition is steadily increasing,...
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RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens
RNA-based vaccines have sparked a paradigm shift in the treatment and prevention of diseases by nucleic acid medicines. There has been a notable...
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Modulation of MicroRNA Expression During In Vitro Chondrogenesis
Since their discovery in 1993, microRNAs (miRNAs) are now recognized as important epigenetic regulators of many mammalian cellular processes...
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The cellular lives of Wolbachia
Wolbachia are successful Gram-negative bacterial endosymbionts, globally infecting a large fraction of arthropod species and filarial nematodes....
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Sperm DNA Fragmentation Testing in Infertility
Sperm DNA fragmentation (SDF) refers to breaks or damage in the DNA strand(s) in sperm cells, which can lead to an increased risk of male...
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Dynamic nucleosome organization after fertilization reveals regulatory factors for mouse zygotic genome activation
Chromatin remodeling is essential for epigenome reprogramming after fertilization. However, the underlying mechanisms of chromatin remodeling remain...
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Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement
PurposePlatelet-rich plasma (PRP) is a remarkable substance, which involves the growth and proliferation of all cell types. As a source of growth...
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Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach
BackgroundGenetic selection in cattle has been directed to increase milk production. This, coupled to the fact that the vast majority of bovine...
