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  1. Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe

    Adenylation domains (A-domains) are responsible for the selective incorporation of carboxylic acid substrates in the biosynthesis of nonribosomal...
    Akimasa Miyanaga, Fumitaka Kudo, Tadashi Eguchi in Non-Ribosomal Peptide Biosynthesis and Engineering
    Protocol 2023
  2. Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms

    Bacterial vaginosis (BV) is the most common vaginal infection worldwide. We developed a peptide nucleic acid (PNA) probe targeting Prevotella bivia ,...

    Lúcia G. V. Sousa, Carina Almeida, ... Nuno Cerca in npj Biofilms and Microbiomes
    Article Open access 23 June 2023
  3. Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP

    Fluorescent in situ hybridization (FISH) enables the visualization of the position and abundance of nucleic acid molecules in fixed cell and tissue...
    Monika W. Perez, Conor K. Camplisson, Brian J. Beliveau in Fluorescence In Situ Hybridization (FISH)
    Protocol 2024
  4. Reinvestigation on primary processes of PSII-dimer from Thermosynechococcus vulcanus by femtosecond pump-probe spectroscopy

    Cyanobacterial photosynthetic apparatus efficiently capture sunlight, and the energy is subsequently transferred to photosystem I (PSI) and II...

    Daisuke Kosumi, Miki Bandou-Uotani, ... Nobuo Kamiya in Photosynthesis Research
    Article 01 January 2024
  5. Rapid identification of Ips sexdentatus (Boerner, 1766) (Curculionidae) from adults and frass with real-time PCR based on probe technology

    A molecular tool has been developed for the molecular identification of Ips sexdentatus (Börner 1776) (Coleoptera Curculionidae Scolytidae), the...

    D. Rizzo, C. G. Zubieta, ... M. Moriconi in Journal of Plant Diseases and Protection
    Article Open access 27 April 2024
  6. A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel

    Background

    Optimum moisture in straw and grain at maturity is important for timely harvesting of wheat. Grain harvested at the right time has reduced...

    Jatinder S. Sangha, Brad Meyer, ... Gaozhi **ao in Plant Methods
    Article Open access 10 June 2024
  7. ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

    Methods that measure the transcriptomic state of thousands of individual cells have transformed our understanding of cellular heterogeneity in...

    Prosenjit Samanta, Samuel F. Cooke, ... Adam Rosenthal in Nature Protocols
    Article 20 May 2024
  8. Development of a MGB probe based qPCR protocol for detecting Pacific cod Gadus macrocephalus in eDNA samples

    The **haeman Bay, located in the South Sea of ​​Korea, is a well-known large-scale Pacific cod ( Gadus macrocephalus ) spawning ground where it migrates...

    Woo-Seok Gwak, Kouji Nakayama in Conservation Genetics Resources
    Article 26 September 2023
  9. Probe-based bacterial single-cell RNA sequencing predicts toxin regulation

    Clonal bacterial populations rely on transcriptional variation across individual cells to produce specialized states that increase fitness....

    Ryan McNulty, Duluxan Sritharan, ... Adam Z. Rosenthal in Nature Microbiology
    Article Open access 03 April 2023
  10. Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples

    A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly ( Lednia tumana ) environmental (e)DNA in water samples. The...

    Patrick R. Hutchins, Jonathan J. Giersch, ... Clint C. Muhlfeld in Conservation Genetics Resources
    Article 03 July 2023
  11. epHero – a tandem-fluorescent probe to track the fate of apoptotic cells during efferocytosis

    The efficient removal of apoptotic cells via efferocytosis is critical for maintaining optimal tissue function. This involves the binding and...

    Sanjna Singh, Julien Bensalem, ... Timothy J. Sargeant in Cell Death Discovery
    Article Open access 17 April 2024
  12. Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes

    The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and...

    Asako Tan, Senthil Murugapiran, ... Daryl M. Gohl in BMC Microbiology
    Article Open access 20 October 2023
  13. DNA-based nanosized functional ruthenium(II) complex as potential phosphorescent probe to track tumor cells

    Identifying tumor cells can be challenging due to cancer’s complex and heterogeneous nature. Here, an efficacious phosphorescent probe that can...

    Jiacheng Wang, Shiqi Gao, ... Wenjie Mei in Molecular Diversity
    Article 15 June 2024
  14. Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens

    The incidence of Mycobacterium tuberculosis (MTB) is increasing due to lack of appropriate diagnostic and therapeutic methods. Therefore, early and...

    Sara Kooti, Sepide Kadivarian, ... Amirhooshang Alvandi in World Journal of Microbiology and Biotechnology
    Article 15 March 2023
  15. Fluorescence Probe Based on Pyrimidine Applied for Rapid Identification of Different Amino Acids

    Abstract

    Rapid identification of different amino acid responses is of great importance in the field of biomedicine. Through the combination of the...

    Article 21 February 2023
  16. Customizable, wireless and implantable neural probe design and fabrication via 3D printing

    This Protocol Extension describes the low-cost production of rapidly customizable optical neural probes for in vivo optogenetics. We detail the use...

    Kyle E. Parker, Juhyun Lee, ... Jordan G. McCall in Nature Protocols
    Article 21 October 2022
  17. Pump-Probe Spectroscopy, Photon Echoes, Two-Dimensional Spectroscopy and Vibrational Wavepackets

    In the last chapter, we used a steady-state treatment to relate the shape of an absorption band to the dynamics of relaxations in the excited state....
    William W. Parson, Clemens Burda in Modern Optical Spectroscopy
    Chapter 2023
  18. The impact of iron (III) on fluorescent dissolved organic matter (fDOM) probe measurements

    Dissolved organic matter (DOM) is ubiquitous in freshwater systems, and fluorescent dissolved organic matter (fDOM) probes have become a popular tool...

    Natalia Malina, Ann Sullivan Ojeda in Aquatic Sciences
    Article 04 February 2023
  19. A real-time PCR assay using locked nucleic acid probe for detection of Acidovorax citrulli

    Watermelon Bacterial Fruit Blotch disease caused by Acidovorax citrulli is an economically important disease to the watermelon plant. The primary...

    Nurhan Öztürk, Hüseyin Basim in Journal of Plant Diseases and Protection
    Article 04 February 2022
  20. Within-field extrapolation away from a soil moisture probe using freely available satellite imagery and weather data

    Recognition of the importance of soil moisture information to the optimisation of water-limited dryland cereal production has led to Australian...

    R. G. V. Bramley, E. M. Perry, ... R. A. Lawes in Precision Agriculture
    Article Open access 02 April 2024
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