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Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe
Adenylation domains (A-domains) are responsible for the selective incorporation of carboxylic acid substrates in the biosynthesis of nonribosomal... -
Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms
Bacterial vaginosis (BV) is the most common vaginal infection worldwide. We developed a peptide nucleic acid (PNA) probe targeting Prevotella bivia ,...
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Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP
Fluorescent in situ hybridization (FISH) enables the visualization of the position and abundance of nucleic acid molecules in fixed cell and tissue... -
Reinvestigation on primary processes of PSII-dimer from Thermosynechococcus vulcanus by femtosecond pump-probe spectroscopy
Cyanobacterial photosynthetic apparatus efficiently capture sunlight, and the energy is subsequently transferred to photosystem I (PSI) and II...
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Rapid identification of Ips sexdentatus (Boerner, 1766) (Curculionidae) from adults and frass with real-time PCR based on probe technology
A molecular tool has been developed for the molecular identification of Ips sexdentatus (Börner 1776) (Coleoptera Curculionidae Scolytidae), the...
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A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel
BackgroundOptimum moisture in straw and grain at maturity is important for timely harvesting of wheat. Grain harvested at the right time has reduced...
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ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets
Methods that measure the transcriptomic state of thousands of individual cells have transformed our understanding of cellular heterogeneity in...
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Development of a MGB probe based qPCR protocol for detecting Pacific cod Gadus macrocephalus in eDNA samples
The **haeman Bay, located in the South Sea of Korea, is a well-known large-scale Pacific cod ( Gadus macrocephalus ) spawning ground where it migrates...
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Probe-based bacterial single-cell RNA sequencing predicts toxin regulation
Clonal bacterial populations rely on transcriptional variation across individual cells to produce specialized states that increase fitness....
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Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples
A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly ( Lednia tumana ) environmental (e)DNA in water samples. The...
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epHero – a tandem-fluorescent probe to track the fate of apoptotic cells during efferocytosis
The efficient removal of apoptotic cells via efferocytosis is critical for maintaining optimal tissue function. This involves the binding and...
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Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes
The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and...
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DNA-based nanosized functional ruthenium(II) complex as potential phosphorescent probe to track tumor cells
Identifying tumor cells can be challenging due to cancer’s complex and heterogeneous nature. Here, an efficacious phosphorescent probe that can...
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Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens
The incidence of Mycobacterium tuberculosis (MTB) is increasing due to lack of appropriate diagnostic and therapeutic methods. Therefore, early and...
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Fluorescence Probe Based on Pyrimidine Applied for Rapid Identification of Different Amino Acids
AbstractRapid identification of different amino acid responses is of great importance in the field of biomedicine. Through the combination of the...
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Customizable, wireless and implantable neural probe design and fabrication via 3D printing
This Protocol Extension describes the low-cost production of rapidly customizable optical neural probes for in vivo optogenetics. We detail the use...
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Pump-Probe Spectroscopy, Photon Echoes, Two-Dimensional Spectroscopy and Vibrational Wavepackets
In the last chapter, we used a steady-state treatment to relate the shape of an absorption band to the dynamics of relaxations in the excited state.... -
The impact of iron (III) on fluorescent dissolved organic matter (fDOM) probe measurements
Dissolved organic matter (DOM) is ubiquitous in freshwater systems, and fluorescent dissolved organic matter (fDOM) probes have become a popular tool...
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A real-time PCR assay using locked nucleic acid probe for detection of Acidovorax citrulli
Watermelon Bacterial Fruit Blotch disease caused by Acidovorax citrulli is an economically important disease to the watermelon plant. The primary...
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Within-field extrapolation away from a soil moisture probe using freely available satellite imagery and weather data
Recognition of the importance of soil moisture information to the optimisation of water-limited dryland cereal production has led to Australian...