Search
Search Results
-
Primer extension refractory PCR: an efficient and reliable genome walking method
Genome walking, a molecular technique for obtaining unknown flanking genomic sequences from a known genomic sequence, has been broadly applied to...
-
Nonenzymatic Template-Directed Primer Extension Using 2′-3′ Cyclic Nucleotides Under Wet-Dry Cycles
RNA World Hypothesis is centred around the idea of a period in the early history of life’s origin, wherein nonenzymatic oligomerization and...
-
A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α–primase
The mechanism by which polymerase α–primase (polα–primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for...
-
Primer Design
An overview of the application of oligonucleotide design is given in relation to applications involving exploratory investigations and diagnostics....
-
Identification of fish species through tRNA-based primer design
BackgroundThe correct establishment of the barcode classification system for fish can facilitate biotaxonomists to distinguish fish species, and it...
-
Tetra-Primer Amplification Refractory Mutation System (T-ARMS)
Single-nucleotide polymorphisms (SNPs), the most abundant genetic variation in the population, have become the molecular marker of choice. Generally,...
-
Allele-Specific Mutation Genoty** with Mismatches in Primer Design
Genoty** technologies for single nucleotide polymorphisms (SNPs) and other mutation types have evolved to become essential tools in various fields....
-
PCR Allele Competitive Extension (PACE)
PCR allele competitive extension (PACE™) is a marker system to analyze single nucleotide polymorphisms (SNPs). As strongly related technologies like...
-
Development and validation of a universal primer pair for the taxonomic and phylogenetic studies of vertebrates
BackgroundRecent studies in the field of molecular identification have described 16S rRNA gene as a highly informative fragment of mitochondrial DNA...
-
Slippage of the Primer Strand in the Primer Extension Reaction with Modified 2'-Deoxyuridine Triphosphates
AbstractAn effect of primer strand slippage was observed in the primer extension reaction due to replacement of thymidine triphosphate for modified...
-
Elucidating the genetic diversity and population structure of international cotton germplasm using inter-primer binding site (iPBS) retrotransposon marker system
Cotton ( Gossypium hirsutum L.) is an important crop throughout the world and is one of the most important industrial raw materials. It is very...
-
primer**n: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR
BackgroundMultiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of...
-
LSQP-DB: a species-specific quantitative PCR primer database for 307 Lactobacillaceae species
Quantitative real-time PCR is widely used to determine absolute abundance of microbes in food fermentation. However, it remains challenges in the...
-
Mutagenic primer-based novel multiplex PCR–RFLP technique to genotype BECN1 SNPs rs10512488 and rs11552192
BackgroundSingle Nucleotide Polymorphisms (SNPs) in candidate autophagy gene BECN1 could influence its functions thereby autophagy process. BECN1 ...
-
Decoding genetic diversity and population structure of Brassica species by inter primer binding site (iPBS) retrotransposon markers
Marker-assisted breeding serves as a potent tool for screening target germplasm, assessing genetic diversity, and determining breeding potential of a...
-
Exploring the genetic diversity and population structure of fenugreek (Trigonella foenum-graecum L.) genotypes through inter-primer binding site (iPBS)-retrotransposon marker system
Fenugreek, a key medicinal-aromatic plant, offers rich bioactive compounds and nutritional value. Its diverse applications in cuisine and...
-
Multiplex on-Chip PCR with Direct Detection of Immobilized Primer Elongation
A variant of multiplex PCR on a chip with direct detection of immobilized primer elongation has been developed. Detection is performed by determining...
-
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2
RNA replication and transcription machinery is an important drug target for fighting against coronavirus. Non-structure protein nsp8 was proposed...
-
Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome
The single-stranded DNA-binding protein gp2.5 of bacteriophage T7 plays myriad functions in the replication of phage genomes. In addition to...
-
The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity
BackgroundA large number of variants have been employed in various medical applications, such as providing medication instructions, disease...
