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Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells
In this chapter, the procedure of isolation and propagation of guinea pig gastric tissue primary cells in cell culture in vitro is presented....
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Primary Isolation of Mycobacterium ulcerans
Primary isolation of Mycobacterium ulcerans is the separation and growth of the bacterium from a mixed population either in clinical specimen or...
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Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization
Leukemia stem cells (LSC) are thought to be the basis of leukemia progression since they are highly resistant to conventional chemotherapy. LSC...
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Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update
In the healthy liver, quiescent hepatic stellate cells (HSCs) are found in the perisinusoidal space (i.e., the space of Dissé) in close proximity to...
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Isolation and Maintenance in Culture of Primary Human Trophoblast from Term Placentae
Trophoblasts are placenta-specific epithelial cells that play an essential role in conducting nutrient, gas, and waste exchange between the fetus and...
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Chondrocyte Isolation and Expansion
Chondrocyte isolation requires a combination of enzymatic and mechanical digestion of cartilaginous tissues in order to release the chondrocytes....
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Isolation of tumor stem-like cells from primary laryngeal squamous cell carcinoma cells (FD-LS-6)
The development of efficient treatments for laryngeal squamous cell carcinoma (LSCC) is hindered by the lack of applicable tumor cell lines and...
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Isolation Techniques for Amoeba
Amoebae are among the most common and abundant protists in all soil types. They can be relatively easily isolated using simple methods when they are...
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Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures
The low number of neural progenitor cells (NPCs) present in the adult and aged primate brains represents a challenge for generating high-yield and...
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Isolation and primary culture of human abdominal aorta smooth muscle cells from brain-dead donors: an experimental model for vascular diseases
Primary cell cultures are essential tools for elucidating the physiopathological mechanisms of the cardiovascular system. Therefore, a primary...
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Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrinder
Primary cells form the basis of modern-day in vitro research analysis tools. Many conventional procedures for generating single-cell suspensions from...
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Isolation of High-Quality Plastids from the Diatom Phaeodactylum tricornutum
Phaeodactylum tricornutum, a model pennate diatom, carries a secondary plastid surrounded by four membranes. Its biological function remains...
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Human Hepatic Stellate Cells: Isolation and Characterization
Liver fibrosis of different etiologies is characterized by activation of hepatic stellate cells (aHSCs) into collagen type I secreting...
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Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications
Primary cell culturePrimary cell cultures is an invaluable method frequently used to overcome challenges associated with in vivoIn vivo experiments....
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Isolation of Human Adipose-Derived Stem Cells
Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine....
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Isolation and Culturing of Cells from Different Tissues
In order to understand the function/defect of any cell, we primarily need to isolate the cell from the provided tissue sample. As isolated cells from...
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An epidemic dynamics model with limited isolation capacity
We consider a modified SIR model with a four-dimensional system of ordinary differential equations to consider the influence of a limited isolation...
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Bacteriophages Isolation and Efficacy Testing
BacteriophagesBacteriophages (phages) are viruses that infect bacteriaBacteria and are the most abundant biological entity on the planet. Phages have...
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Isolation and Identification of Edwardsiellosis-Causing Microorganism
Isolation and identification of pathogenic bacteria are crucial for aquatic health management. Identifying the correct pathogenic bacteria at the...
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Isolation of Mitochondria from Murine Skeletal Muscle
Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body’s metabolic homeostasis,...