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Heterologous Gene Expression in Pichia pastoris: Success Stories and Commercial Ventures
Heterologous protein production in the methylotrophic yeast, Pichia pastoris, has gained immense popularity owing to the growing demands for...
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Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris
Cisternal stacking is reversible, initiated at the “cis” side of the Golgi, and gets undone at the “trans” side in a continuous cycle in tune with...
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A simple and effective method to remove pigments from heterologous secretory proteins expressed in Pichia pastoris
Pichia pastoris is a popular yeast host for high-level heterologous expression of proteins on an industrial scale owing to its reliable expression,...
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Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system
Yeasts serve as exceptional hosts in the manufacturing of functional protein engineering and possess industrial or medical utilities. Considerable...
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Production of Single-Domain Antibodies in Pichia pastoris
Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic...
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The Expression of Recombinant Human Hepatocyte Growth Factor in Pichia pastoris
AbstractThe aim of this study was to construct a stable and efficient eukaryotic expression system for the secretion of biologically active...
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Characterization of Prolyl-4-Hydroxylase Substrate Specificity Using Pichia pastoris as an Efficient Eukaryotic Expression System
The use of eukaryotic expression systems facilitates the heterologous expression of complex eukaryotic proteins in their post-translationally...
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Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris
AbstractAromatic secondary metabolites are widely used in various industries, including the nutraceutical, dietary supplement, and pharmaceutical...
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Characterization and High Expression of a Novel Alkalinegh11 Xylanase in Pichia pastoris
AbstractA novel alkaline xylanase gene ( xyn AI) from Bacillus amyloliquefaciens was highly expressed in Pichia pastoris , and the enzyme was purified...
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Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris
AbstractHyaluronidases catalyze the degradation of hyaluronan (HA), which is finding rising applications in medicine, cosmetic, and food industries....
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Recombinant expression of IGF-1 and LR3 IGF-1 fused with xylanase in Pichia pastoris
AbstractInsulin-like growth factor-1 (IGF-1) is a pleiotropic protein hormone and has become an attractive therapeutic target because of its multiple...
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Improvement of the recombinant phytase expression by intermittent feeding of glucose during the induction phase of methylotrophic yeast Pichia pastoris
PurposeFor growth of methylotrophic yeast, glycerol is usually used as a carbon source. Glucose is used in some cases, but not widely consumed due to...
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Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host
Fungal natural products have extensive biological activities, and thus have been largely commercialized in the pharmaceutical, agricultural, and food...
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De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris
PurposePichia pastoris is well known for its ability to produce short and low-immunogenic humanized glycosyl chains onto recombinant glycoproteins,...
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Heterologous expression of pediocin PA-1 in Pichia pastoris: cloning, expression, characterization, and application in pork bologna preservation
ObjectivePediocin PA-1, an antimicrobial peptide derived from Pediococcus acidilactici PAC1.0, has a potential application as a food preservative...
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Rapid Screening for High Expressing Multicopy Recombinants and Enhanced Epidermal Growth Factor (EGF) Protein Production Using Pichia Pastoris
Epidermal growth factor (EGF) is a highly effective stimulator which promotes cell proliferation and this has led it to being proposed as a...
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Effect and mechanism of signal peptide and maltose on recombinant type III collagen production in Pichia pastoris
AbstractRecombinant type III collagen plays an important role in cosmetics, wound healing, and tissue engineering. Thus, increasing its production is...
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Medium optimization for enhanced production of recombinant lignin peroxidase in Pichia pastoris
ObjectivesDifferent cultivation conditions and parameters were evaluated to improve the production and secretion of a recombinant Phanerochaete...
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Optimization of a Purification Method for the Recombinant Platelet-Derived Growth Factor rhPDGF-BB Expressed in the Methylotrophic Yeast Pichia Pastoris
Recombinant human platelet-derived growth factor rhPDGF-BB is one of the major cytokines that has been approved for medical use. Medical drug...
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Design of a novel switchable antibody display system in Pichia pastoris
AbstractYeast surface display (YSD) has been shown to represent a powerful tool in the field of antibody discovery and engineering as well as for...
