Search
Search Results
-
Functional and structural diversification of incomplete phosphotransferase system in cellulose-degrading clostridia
Carbohydrate utilization is critical to microbial survival. The phosphotransferase system (PTS) is a well-documented microbial system with a...
-
Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase
Despite their low abundance, phosphoinositides play a central role in membrane traffic and signalling. PtdIns(3,4,5)P 3 and PtdIns(3,4)P 2 are uniquely...
-
Structure of the human GlcNAc-1-phosphotransferase αβ subunits reveals regulatory mechanism for lysosomal enzyme glycan phosphorylation
Vertebrates use the mannose 6-phosphate (M6P)-recognition system to deliver lysosomal hydrolases to lysosomes. Key to this pathway is N -acetylglucosam...
-
Transcriptome analysis of Magnaporthe oryzae reveals the role of histidine phosphotransferase in pathogenesis and stress adaptation
Magnaporthe oryzae is notorious for its efficient evolution as a plant pathogen. Analysis of well organised fungal pathogenic mechanisms would enable...
-
Unveiling the fructose metabolism system in Staphylococcus aureus: insights into the regulatory role of FruR and the FruRKT operon in bacterial fitness
BackgroundThe utilization of fructose as a carbon source and energy provider plays a crucial role in bacterial metabolism. Additionally, fructose...
-
-
Transcriptional modulation of the global regulator CodY using a conditional CRISPRi system in Bacillus licheniformis
Bacillus licheniformis is an industrially significant microorganism known for its broad carbon source utilization compared to other bacteria....
-
Efficient genome editing in grapevine using CRISPR/LbCas12a system
Clustered regularly interspaced short palindromic repeats (CRISPR) /Cas12a system, also known as CRISPR/Cpf1, has been successfully harnessed for...
-
Stable transformation of the unicellular lichenic green alga Coccomyxa solorinae-saccatae via electroporation
In this study, we have demonstrated the applicability of electroporation for the stable nuclear transformation of Coccomyxa solorinae-saccatae . An...
-
Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector
The CRISPR/Cas9 technique for rice genome engineering is gaining momentum and requires a precise gene delivery system. For rice and other crop...
-
Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin
Metagenomic-based studies have predicted an extraordinary number of potential antibiotic-resistance genes (ARGs). These ARGs are hidden in various...
-
An efficient Agrobacterium tumefaciens-mediated transformation system of Taraxacum kok-saghyz Rodin (Russian dandelion)
Taraxacum kok-saghyz Rodin (TK) is an excellent alternative to Hevea brasiliensis because of its high yield of latex, wide growth range, and short...
-
A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries
Transient transformation is extremely useful for rapid in vivo assessment of gene function, especially for fruit-related genes. Grape berry, while an...
-
Identification and genome-wide analysis provide insights into the genetic diversity and biotechnological potentials of novel cold-adapted Acinetobacter strain
Extreme cold environments, such as polar regions or high-altitude mountains, are known for their challenging conditions including low temperatures,...
-
An effective transient expression system for gene function identification in Lotus japonicus
Transient transformation is a notably efficient and expeditious method for obtaining foreign gene expression products, making it a prominent avenue...
-
Leveraging quorum sensing system for automatic coordination of Escherichia coli growth and lactic acid biosynthesis
PurposeOverproduction of desired metabolites usually sacrifices cell growth. Here we report that quorum sensing (QS) can be exploited to coordinate...
-
-
Characterization of phyllosphere endophytic lactic acid bacteria reveals a potential novel route to enhance silage fermentation quality
The naturally attached phyllosphere microbiota play a crucial role in plant-derived fermentation, but the structure and function of phyllosphere...
-
Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria
Metabolic cross-feeding is a pervasive microbial interaction type that affects community stability and functioning and directs carbon and energy...
-
Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system
The endophytic fungus Berkleasmium sp. Dzf12 that was isolated from Dioscorea zingiberensis , is a proficient producer of palmarumycins, which are...
