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Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins
Bacterial outer membrane vesicles (OMVs) can be selectively enriched with one or more outer membrane proteins to allow the biophysical...
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Identification and characterisation of a major outer membrane protein from Methylacidiphilum fumariolicum SolV
The outer membrane (OM) protects Gram-negative bacteria against a hostile environment. The proteins embedded in the OM fulfil a number of tasks that...
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OmpA is Involved in the Early Response of Escherichia coli to Antibiotics as a Hub Outer Membrane Protein
AbstractThe environmental pollution caused by antibiotics is becoming more and more serious. Outer membrane proteins (OMPs) play key roles in the...
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Generation of unfolded outer membrane protein ensembles defined by hydrodynamic properties
Outer membrane proteins (OMPs) must exist as an unfolded ensemble while interacting with a chaperone network in the periplasm of Gram-negative...
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Defining Membrane Protein Localization by Isopycnic Density Gradients
Bacterial membrane proteins account for around one-third of the proteome in many species and can represent much more than half of the mass of the...
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Stress-Based Screening for Compounds That Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria
Biogenesis of the outer membrane (OM) of Gram-negative bacteria involves two processes essential for growth, that is, the insertion of β-barrel outer...
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Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes
The type 9 secretion system (T9SS) is a recently discovered machinery that both transports cargo proteins across the Gram-negative bacterial outer...
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Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System
The SpyCatcher-SpyTag system has become a popular and versatile tool for protein ligation. It is based on a small globular protein (SpyCatcher) that...
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Maintenance of bacterial outer membrane lipid asymmetry: insight into MlaA
The outer membrane (OM) of Gram-negative bacteria acts as an effective barrier to protect against toxic compounds. By nature, the OM is asymmetric...
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Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes
β-barrel membrane proteins populate the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts, playing significant roles in...
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Oral administration of recombinant outer membrane protein A-based nanovaccine affords protection against Aeromonas hydrophila in zebrafish
Aeromonas hydrophila , an opportunistic warm water pathogen, has always been a threat to aquaculture, leading to substantial economic losses....
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Tackling the outer membrane: facilitating compound entry into Gram-negative bacterial pathogens
Emerging resistance to all available antibiotics highlights the need to develop new antibiotics with novel mechanisms of action. Most of the...
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Immunopotential of Pasteurella multocida bivalent outer membrane protein-based vaccine entrapped in aluminum hydroxide nanoparticles
Swine pasteurellosis is one of the most economically important diseases of pig caused by Pasteurella multocida ( P. multocida ) capsular types A and D....
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Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens
AbstractBacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived...
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Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry
Chemical crosslinking-mass spectrometry (XL-MS) is an established tool that can be used to study the architecture and dynamics of proteins and...
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Membrane Glycoprotein (M-Protein)
SARS-CoV-2 membrane proteinMembrane protein (M-proteinM-protein) performs a variety of critical functions in virus infection cycle. In this chapter...
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Pore-Forming VDAC Proteins of the Outer Mitochondrial Membrane: Regulation and Pathophysiological Role
AbstractVoltage-dependent anion channels (VDAC1-3) of the outer mitochondrial membrane are a family of pore-forming β-barrel proteins that carry out...
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Pro-inflammatory cytokines and reproductive hormone responses in bucks post-challenge with Mannheimia haemolytica A2 and its outer membrane protein
The lipopolysaccharide (LPS) endotoxin and outer membrane protein (OMP) are among the virulence factors of Gram-negative bacteria responsible for...
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Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages
AbstractPasteurella multocida is an important bacterial pathogen that can cause diseases in both animals and humans. Its elevated morbidity and...
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Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria depends on delivery of unfolded OMPs to the...
