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The effect of mixtures of Bacillus thuringiensis-based insecticide and multiple nucleopolyhedrovirus of Lymantria dispar L. in combination with an optical brightener on L. dispar larvae
This study evaluated the efficacy of the commercially available insecticide Lepidocide based on Bacillus thuringiensis var. kurstaki and Lymantria...
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Immunodetection of Cell Wall Components in Studies on Cell Wall Rebuilding in Fagopyrum esculentum and Fagopyrum tataricum
Immunocytochemical studies of the cell wall are used to visualize specific epitopes of pectins, arabinogalactan proteins, hemicelluloses, extensins,... -
Lectin Receptor-Like Protein Kinase OsNRFG6 is Required for Embryo Sac Development and Fertilization in Neo-Tetraploid Rice
Great yield-enhancing prospects of autotetraploid rice was restricted by various polyploidy-induced reproductive dysfunction. To surmount these...
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Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMap
Retrieving the complex responses of individual cells in the native three-dimensional tissue context is crucial for a complete understanding of tissue...
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A diffusible small-RNA-based Turing system dynamically coordinates organ polarity
The formation of a flat and thin leaf presents a developmentally challenging problem, requiring intricate regulation of adaxial–abaxial (top–bottom)...
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Cortical microtubule dynamics during reaction wood formation ensures context-appropriate cellulose microfibril angle in woody trees
Key messageCortical microtubule arrays are the primary mechanism for guiding the re-orientation of cellulose microfibrils and determining MFA in...
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Rapid Visual Detection of Spiroplasma eriocheiris by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
In recent years, a new type of Spiroplasma has been found that can cause “tremor disease” of the Chinese mitten crab Eriocheir sinensis . The outbreak...
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Production improvement and photoprotection of melanin produced by Bacillus thuringiensis
In our previous research, we constructed a Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae; Bt) strain (BMB171Δ hmgA ), in which the loss of...
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An environmentally safe and tolerant microbial insecticide utilizing Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR)
BackgroundBaculoviruses are significant biocontrol agents of pests in agriculture; however, ultraviolet light B (UV-B) and high temperature...
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Reconstruction pattern of the cell wall in Fagopyrum protoplast-derived hybrid cells
The cell wall rebuilding is one of the first stage of protoplast development that enables further mitotic divisions and differentiation. Therefore,...
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A somatic genetic clock for clonal species
Age and longevity are key parameters for demography and life-history evolution of organisms. In clonal species, a widespread life history among...
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Means to Quantify Vascular Cell File Numbers in Different Tissues
Oriented cell divisions are crucial throughout plant development to define the final size and shape of organs and tissues. As most of the tissues in... -
Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation
Inducing the differentiation of specific cell type(s) synchronously and on-demand is a great experimental system to understand the sequential... -
Inoculation with Arbuscular Mycorrhizal Fungi Reinforces Tea Plant’s Tolerance to Salinity
A major stress factor that can seriously limit plant growth and productivity is the soil degradation caused by salinity. Arbuscular mycorrhizal fungi...
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Improved clearing method contributes to deep imaging of plant organs
Tissue clearing methods are increasingly essential for the microscopic observation of internal tissues of thick biological organs. We previously...
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Molecular characterization and biological activity of native Spodoptera littoralis nucleopolyhedrovirus isolate
BackgroundSpodoptera littoralis nucleopolyhedrovirus (SpliNPV) is considered a promising biocontrol agent that can be used for the effective control...
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Novel findings about the mode of action of the antifungal protein PeAfpA against Saccharomyces cerevisiae
AbstractAntifungal proteins (AFPs) from filamentous fungi offer the potential to control fungal infections that threaten human health and food...
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Fluorochromes for the Study of the Cell Features
This chapter is dedicated to fluorochromes used in the study of cellular characteristics. It divides them according to the characteristics studied,... -
Microtubule self-organisation during seed germination in Arabidopsis
BackgroundUpon water uptake and release of seed dormancy, embryonic plant cells expand, while being mechanically constrained by the seed coat....
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PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr
At present, a cooperative process hypothesis is used to explain the supply of enzyme (class III peroxidases and/or laccases) and substrates during...