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Split complementation of base editors to minimize off-target edits
Base editors (BEs) empower the efficient installation of beneficial or corrective point mutations in crop and human genomes. However, conventional...
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Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors depend on accurate prediction of on-target activity and...
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Undetectable off-target effects induced by FokI catalytic domain in mouse embryos
The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of...
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Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing
The continued development of novel genome editors calls for a universal method to analyze their off-target effects. Here we describe a versatile...
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Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity
Genome editing with CRISPR-associated (Cas) proteins holds exceptional promise for “correcting” variants causing genetic disease. To realize this...
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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is crucial for genome editing applications, but currently...
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Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short...
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Comprehensive computational analysis of epigenetic descriptors affecting CRISPR-Cas9 off-target activity
BackgroundA common issue in CRISPR-Cas9 genome editing is off-target activity, which prevents the widespread use of CRISPR-Cas9 in medical...
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Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag
Prime editors have a broad range of potential research and clinical applications. However, methods to delineate their genome-wide editing activities...
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Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine...
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In situ-formable, dynamic crosslinked poly(ethylene glycol) carrier for localized adeno-associated virus infection and reduced off-target effects
The adeno-associated virus (AAV) is a potent vector for in vivo gene transduction and local therapeutic applications of AAVs, such as for skin...
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The antipsychotic medications aripiprazole, brexpiprazole and cariprazine are off-target respiratory chain complex I inhibitors
Antipsychotic drugs are the mainstay of treatment for schizophrenia and provide adjunct therapies for other prevalent psychiatric conditions,...
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Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment
Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability...
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Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors
Programmable cytosine base editors show promising approaches for correcting pathogenic mutations; yet, their off-target effects have been of great...
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Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches
We present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and...
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