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Nucleic Acid Amplification Free-QCM-DNA Biosensor for Burkholderia pseudomallei Detection
Burkholderia pseudomallei is a gram-negative bacterium that causes the infectious disease melioidosis, a disease that can still be fatal despite...
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Development of diagnostic tests for pathogen identification and detection of antimicrobial resistance on WHO global priority pathogens using modular real-time nucleic acid amplification test
BackgroundConcerns regarding antimicrobial resistance (AMR) have resulted in the World Health Organization (WHO) designating so-called global...
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Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a
Vibrio alginolyticus (V. alginolyticus) is a common pathogen in the ocean. In addition to causing serious economic losses in aquaculture, it can also...
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Optimization of a rapid and sensitive nucleic acid lateral flow biosensor for hepatitis B virus detection
Background and objectiveThe utilization of direct amplification of nucleic acid from lysate has attracted interest in the advancement of...
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Comparison between nucleic acid amplification tests, antigen immunofluorescence assay, and in vitro infectivity in SARS-CoV-2 diagnosis
The number of SARS-CoV-2 detection tests requested to the laboratories has dramatically increased together with an urgent need to release reliable...
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Risk factors for recurrent positive results of the nucleic acid amplification test for COVID-19 patients: a retrospective study
Positive retests of COVID-19 represent a public health concern because of the increased risk of transmission. This study explored whether factors...
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An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review
Precise and timely diagnosis of plant viruses is a prerequisite for the implementation of efficient management strategies, considering factors like...
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Improving the specificity of nucleic acid detection with endonuclease-actuated degradation
Nucleic acid detection is essential for numerous biomedical applications, but often requires complex protocols and/or suffers false-positive...
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Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a
AbstractEarly diagnosis of the African swine fever virus (ASFV) is the main preventive measure for ASFV. Here, we developed a fluorescent biosensor...
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Research progress on nucleic acid detection and genome editing of CRISPR/Cas12 system
PurposeThis work characterizes the applications of CRISPR/Cas12 system, including nucleic acid detection, animal, plant and microbial genome editing.
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Loop-Mediated Isothermal Amplification: From Theory to Practice
AbstractIncreasing the accuracy of pathogen identification and reducing the duration of analysis remain relevant for modern molecular diagnostics up...
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Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes
Detection of nucleic acid amplification has typically required sophisticated laboratory instrumentation, but as the amplification techniques have...
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Screening and identification of DNA nucleic acid aptamers against F1 protein of Yersinia pestis using SELEX method
BackgroundYersinia pestis is a bacterium that causes the disease plague. It has caused the deaths of many people throughout history. The bacterium...
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Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
Prorocentrum lima , a widely distributed dinoflagellate known for its production of harmful biotoxins, poses a significant threat to humans,...
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Rapid detection of porcine sapelovirus by reverse transcription recombinase polymerase amplification assay
BackgroundPorcine Sapelovirus (PSV) infection has been confirmed in pigs worldwide, mostly asymptomatic, but in some cases, it can lead to...
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Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples
Bloodstream infection (BSI) caused by bacteria is highly pathogenic and lethal, and easily develops whole-body inflammatory state. Immediate...
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Peptide Nucleic Acid (PNA) Clamps to Reduce Co-amplification of Plant DNA During PCR Amplification of 16S rRNA Genes from Endophytic Bacteria
High-throughput sequencing of universal bacterial 16S rRNA gene (16S rDNA) amplicons is a routine method for characterizing bacterial diversity in a...
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Novel helix loop-mediated isothermal amplification (HAMP) assay for colorimetric detection of Staphylococcus aureus in milk
Staphylococcus aureus is an important and leading cause of foodborne diseases worldwide. Prompt detection and recall of contaminated foods are...
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Highly Sensitive Detection of Human Pluripotent Stem Cells by Loop-Mediated Isothermal Amplification
For safe regenerative medicines, contaminated or remaining tumorigenic undifferentiated cells in cell-derived products must be rigorously assessed...
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Genomic DNA Extraction from Different Human Tissue Sample: Urine, Oral Gargle, Blood, and Cervical for Real Time Amplification by qPCR
Molecular technologies have been a driver of rapid paradigm shift in the field of scientific research and clinical applications. Adequate and pure...
