Search
Search Results
-
DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity
Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR–Cas, detect and cleave invader nucleic acids using complementary guides...
-
Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity
BackgroundThe CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system....
-
Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
Type V and type VI CRISPR–Cas systems have been shown to cleave nonspecific single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) in trans , but...
-
Monitoring Nuclease Activity by X-Ray Scattering Interferometry Using Gold Nanoparticle-Conjugated DNA
The biologically critical, exquisite specificity and efficiency of nucleases, such as those acting in DNA repair and replication, often emerge in the...
-
Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for...
-
MIF promotes neurodegeneration and cell death via its nuclease activity following traumatic brain injury
Traumatic brain injury (TBI), often induced by sports, car accidents, falls, or other daily occurrences, is a primary non-genetically related risk...
-
Molecular and structural basis of an ATPase-nuclease dual-enzyme anti-phage defense complex
Coupling distinct enzymatic effectors emerges as an efficient strategy for defense against phage infection in bacterial immune responses, such as the...
-
Structural and functional analyses of Burkholderia pseudomallei BPSL1038 reveal a Cas-2/VapD nuclease sub-family
Burkholderia pseudomallei is a highly versatile pathogen with ~25% of its genome annotated to encode hypothetical proteins. One such hypothetical...
-
Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease
Nuclease-mediated editing of heteroplasmic mitochondrial DNA (mtDNA) seeks to preferentially cleave and eliminate mutant mtDNA, leaving wild-type...
-
APEX1 Nuclease and Redox Functions are Both Essential for Adult Mouse Hematopoietic Stem and Progenitor Cells
Self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) are carefully controlled by extrinsic and intrinsic factors, to...
-
Application of DETECTR for Selective Detection of Bacterial Phytopathogen Dickeya solani Using Recombinant CRISPR-Nuclease Cas12a Obtained by Single-Stage Chromatographic Purification
AbstractThe work demonstrates that recombinant CRISPR-nuclease Cas12a, purified after heterologous expression with a simplified method using...
-
Expanding the list of sequence-agnostic enzymes for chromatin conformation capture assays with S1 nuclease
This study presents a novel approach for map** global chromatin interactions using S1 nuclease, a sequence-agnostic enzyme. We develop and outline...
-
Construction of a Z-DNA-Specific Recombinant Nuclease Zαα-FOK for Conformation Studies
Development of FokI-based engineered nucleases has been a platform technology that enables creation of novel sequence-specific nucleases as well as...
-
Transmembrane nuclease NUMEN/ENDOD1 regulates DNA repair pathway choice at the nuclear periphery
Proper repair of DNA damage lesions is essential to maintaining genome integrity and preventing the development of human diseases, including cancer....
-
Effect of Zn2+ Ions on Acid Nuclease Activity in Freshwater Mollusks
AbstractThe effect of increased concentrations of Zn 2+ ions on the activity of the complex of acid deoxyribonucleases (DNases) and ribonucleases...
-
Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a
AbstractGenome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies...
-
A shape-shifting nuclease unravels structured RNA
RNA turnover pathways ensure appropriate gene expression levels by eliminating unwanted transcripts. Dis3-like 2 (Dis3L2) is a 3′–5′ exoribonuclease...
-
Targeted Nuclease Approaches for Map** Native R-Loops
R-loops are three-stranded, DNA:RNA hybrid-containing structures that form naturally throughout the genome as a consequence of transcription....
-
Improvement of duplex-specific nuclease salt tolerance by fusing DNA-binding domain of DNase from an extremely halotolerant bacterium Thioalkalivibrio sp. K90mix
Salt tolerance is an important property of duplex-specific nuclease (DSN). DSN with high salt tolerance can be more widely used in genetic...
-
