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Nuclear Pore Complex-Regulated Transcription and Memory
The nuclear pore complex (NPC) stands as a marvel of cellular architecture that orchestrates the bidirectional traffic of macromolecules between the...
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The Nuclear Pore Complex Methods and Protocols
This volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble...
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Assembly and Disassembly of the Nuclear Pore Complex: A View from the Structural Side
Abstract —Nucleocytoplasmic exchange in the cell occurs through the nuclear pore complexes (NPCs). NPCs are large multiprotein complexes with...
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Nuclear Pore Complex Assembly Using Xenopus Egg Extract
Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ...
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Nucleoporin downregulation modulates progenitor differentiation independent of nuclear pore numbers
Nucleoporins (NUPs) comprise nuclear pore complexes, gateways for nucleocytoplasmic transport. As primary human keratinocytes switch from the...
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Proxiome assembly of the plant nuclear pore reveals an essential hub for gene expression regulation
The nuclear pore complex (NPC) is vital for nucleocytoplasmic communication. Recent evidence emphasizes its extensive association with proteins of...
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Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex
Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR)...
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Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans
C. elegans is a well-characterized and relatively simple model organism, making it attractive for studying nuclear pore complex proteins in cell and...
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The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis
Biogenesis of nuclear pore complexes (NPCs) includes the formation of the permeability barrier composed of phenylalanine-glycine-rich nucleoporins...
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Nuclear pore protein POM121 regulates subcellular localization and transcriptional activity of PPARγ
Manipulation of the subcellular localization of transcription factors by preventing their shuttling via the nuclear pore complex (NPC) emerges as a...
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Cryo-EM structure of the inner ring from the Xenopus laevis nuclear pore complex
Nuclear pore complex (NPC) mediates nucleocytoplasmic shuttling. Here we present single-particle cryo-electron microscopy structure of the inner ring...
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Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins
Posttranslational modifications and in particular ubiquitylationUbiquitylation and SUMOylationSUMOylation of the nuclear pore complex (NPC), have...
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Validation of Nuclear Pore Complex Protein–Protein Interactions by Transient Expression in Plants
The nuclear pore complex (NPC) is the largest protein complex, consisting of multiple copies of over 30 different nucleoporins. The interactions...
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Live CLEM Imaging of Tetrahymena to Analyze the Dynamic Behavior of the Nuclear Pore Complex
TetrahymenaTetrahymena is a fascinating organism for studying the nuclear pore complex because it has two structurally and functionally distinct...
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Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex
Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular...
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Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans
In eukaryotic cells the nuclear envelope encloses the genome separating it from the rest of the cell. Nuclear pore complexes are large multi protein...
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Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts
The nuclear pore complex (NPC) is the conduit in the nuclear envelope through which proteins and RNA are transported between the cytoplasm and...
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Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes
Studying protein complexes in vitro requires the production of a relatively pure sample that maintains the full complement, native organization, and...