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  1. Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with Prostate Cancer

    Nuclear magnetic resonance (NMR) spectroscopy enables the detection and the quantification of a large range of molecules, including...
    Dalia Ahmed, Stefano Cacciatore, Luiz Fernando Zerbini in Metabolic Reprogramming
    Protocol 2023
  2. Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonance

    Carrier proteins (CPs) are central actors in nonribosomal peptide synthetases (NRPSs) as they interact with all catalytic domains, and because they...
    Neeru Arya, Kenneth A. Marincin, Dominique P. Frueh in Non-Ribosomal Peptide Biosynthesis and Engineering
    Protocol 2023
  3. Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography–Mass Spectrometry

    Extracellular vesicle (EV) release and their content are influenced by diverse clinical conditions. EVs participate in inter-cellular communication...
    Marta Martin-Lorenzo, Dolores Molero, Gloria Alvarez-Llamas in Cell-Secreted Vesicles
    Protocol 2023
  4. Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD NMR) Spectroscopy

    Saturation transfer difference (STD) nuclear magnetic resonance (NMR) spectroscopy is an established technique for detecting and characterizing the...
    Ruslan Nedielkov, Heiko M. Möller in Cell-Wide Identification of Metabolite-Protein Interactions
    Protocol 2023
  5. The Complementarity of Nuclear Magnetic Resonance and Native Mass Spectrometry in Probing Protein-Protein Interactions

    Nuclear magnetic resonance (NMR) and native mass spectrometry (MS) are mature physicochemical techniques with long histories and important...
    Chapter 2024
  6. Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy

    Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria. The glycerol phosphate...
    Jeanine Rismondo, Angelika GrĂĽndling in The Bacterial Cell Wall
    Protocol 2024
  7. Determination of nuclear magnetic resonance surface relaxivity for the macropore system from wood cell lumen

    To determine the surface relaxivity of pores plays a vital role in the application of time-domain nuclear magnetic resonance (TD-NMR) technology to...

    Zhi **, Yue** Fu, ... Zhen Zeng in Journal of Wood Science
    Article Open access 11 January 2024
  8. Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans

    Glycosaminoglycans (GAGs) are sulfated glycans of complex structure and multiple biological actions. They are composed of disaccharide repeating...
    Vitor H. Pomin, Barbara Mulloy in Glycosaminoglycans
    Protocol 2022
  9. Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy

    To understand flagella-driven motility of bacteria, it is important to understand the structure and dynamics of the flagellar motor machinery. We...
    Tatsuro Nishikino, Yohei Miyanoiri in Bacterial and Archaeal Motility
    Protocol 2023
  10. Solution-state nuclear magnetic resonance spectroscopy of crystalline cellulosic materials using a direct dissolution ionic liquid electrolyte

    Owing to its high sustainable production capacity, cellulose represents a valuable feedstock for the development of more sustainable alternatives to...

    Lukas Fliri, Katja Heise, ... Alistair W. T. King in Nature Protocols
    Article 26 May 2023
  11. Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR

    Pathogen proliferation and virulence depend on available nutrients, and these vary when the pathogen moves from outside of the host cell...
    Ke-Chuan Wang, Pernille Rose Jensen in Intracellular Pathogens
    Protocol 2024
  12. NMR Analysis of Mammalian Glycolipids

    Mammalian glycolipids play a variety of roles, often coupled with interactions with endogenous and exogenous molecules. The interactions can induce...
    Yoshiki Yamaguchi in Glycolipids
    Protocol 2023
  13. Nuclear Magnetic Resonance Spectroscopy to Analyse Protein Folding and Dynamics

    Proteins are nanoscale machines that perform all the work in living systems. Their function depends on their three-dimensional (3D) structure. These...
    Nikita V. Saibo, Soumendu Boral, ... Soumya De in Protein Folding Dynamics and Stability
    Chapter 2023
  14. Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

    Besides the historical and traditional use of nuclear magnetic resonance (NMR) spectroscopy as a structure elucidation tool for proteins and...
    Caroline Mauve, CĂ©line Oury, ... Michael Hodges in Photorespiration
    Protocol 2024
  15. Nuclear Magnetic Resonance-Guided Structural Analysis of Moderate-Affinity Protein Complexes with Intrinsically Disordered Polypeptides

    Binding affinity of an individual binding site of an intrinsically disordered protein for its folded partner may be moderate. In such cases, a...
    Dmitri Tolkatchev, Garry E. Smith, Alla S. Kostyukova in Advanced Methods in Structural Biology
    Protocol 2023
  16. Metabolic biomarkers using nuclear magnetic resonance metabolomics assay for the prediction of aging-related disease risk and mortality: a prospective, longitudinal, observational, cohort study based on the UK Biobank

    The identification of metabolic biomarkers for aging-related diseases and mortality is of significant interest in the field of longevity. In this...

    Jie Lian, Varut Vardhanabhuti in GeroScience
    Article 30 August 2023
  17. sRNA Structural Modeling Based on NMR Data

    Small non-coding RNAs (sRNAs) play vital roles in gene expression regulation and RNA interference. To comprehend their molecular mechanisms and...
    Pengzhi Wu, Lingna Yang in Bacterial Regulatory RNA
    Protocol 2024
  18. SDS-induced hexameric oligomerization of myotoxin-II from Bothrops asper assessed by sedimentation velocity and nuclear magnetic resonance

    We report the solution behavior, oligomerization state, and structural details of myotoxin-II purified from the venom of Bothrops asper in the...

    Amy Henrickson, Tony Montina, ... Borries Demeler in European Biophysics Journal
    Article 20 May 2023
  19. Nuclear Magnetic Resonance Spectroscopy in Food Analysis

    Nuclear magnetic resonance (NMR) spectroscopy is a powerful spectroscopic technique, conventionally used for structure verification, elucidation, and...
    Ghulam Mustafa Kamal, Jalal Uddin, ... Abdullah Ijaz Hussain in Techniques to Measure Food Safety and Quality
    Chapter 2021
  20. Bone marrow plasma metabonomics of idiopathic acquired aplastic anemia patients using 1H nuclear magnetic resonance spectroscopy

    Introduction

    Idiopathic acquired aplastic anemia (AA) is a bone marrow failure disorder where aberrant T-cell functions lead to depletion of...

    Jyotika Srivastava, Rimjhim Trivedi, ... Chandra P. Chaturvedi in Metabolomics
    Article 17 November 2023
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