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Expansion Microscopy of Plant Cells (PlantExM)
Expansion microscopy (ExM) achieves super-resolution imaging without the need for sophisticated super-resolution microscopy hardware through a...
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More than double the fun with two-photon excitation microscopy
For generations researchers have been observing the dynamic processes of life through the lens of a microscope. This has offered tremendous insights...
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Characterization of Complexes and Supramolecular Structures by Electron Microscopy
Recent advancements in cryo-electron microscopy (cryo-TEM) have enabled the determination of structures of macromolecular complexes at near-atomic...
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Structural Analysis of Protein Complexes by Cryo-Electron Microscopy
Structural studies of bio-complexes using single particle cryo-Electron Microscopy (cryo-EMCryo- electron microscopy (cryo-EM)) is nowadays a...
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Digital Holographic Microscopy to Assess Cell Behavior
Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required...
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GelMap: intrinsic calibration and deformation map** for expansion microscopy
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy by physically expanding biological specimen...
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Expansion microscopy applied to mono- and dual-species biofilms
Expansion microscopy (ExM) is a new super-resolution technique based on embedding the biological sample within a hydrogel and its physical expansion...
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Studying Structure and Functions of Nucleosomes with Atomic Force Microscopy
AbstractChromatin is an epigenetic platform for implementation of DNA-dependent processes. Nucleosome, as a basic level of chromatin compaction,...
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Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images
Microscopy-based spatially resolved omic methods are transforming the life sciences. However, these methods rely on high numerical aperture...
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Technical advances in high-speed atomic force microscopy
It has been 30 years since the outset of develo** high-speed atomic force microscopy (HS-AFM), and 15 years have passed since its establishment in...
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Revealing Ultrastructural Analysis of Starch Using Scanning Electron Microscopy
Starch serves as the main source of energy and carbohydrates in human nutrition. It has a big part in plant foods and offers a lot of desirable...
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Computational biophysics of atomic force microscopy—an IUPAB-sponsored workshop
Atomic Force Microscopy (AFM) is a structural determination technique that involves ‘prodding’ surfaces with a nanometer sized needle with...
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Second harmonic generation microscopy: a powerful tool for bio-imaging
Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the...
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ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy
BackgroundLocalization-based super-resolution microscopy resolves macromolecular structures down to a few nanometers by computationally...
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Fast detection of slender bodies in high density microscopy data
Computer-aided analysis of biological microscopy data has seen a massive improvement with the utilization of general-purpose deep learning...
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Surface photovoltage microscopy for map** charge separation on photocatalyst particles
Solar-driven photocatalytic reactions offer a promising route to clean and sustainable energy, and the spatial separation of photogenerated charges...
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Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity
The highly complex structure of the brain requires an approach that can unravel its connectivity. Using volume electron microscopy and a dedicated...
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Simulating biological surface dynamics in high-speed atomic force microscopy experiments
High-speed atomic force microscopy (HSAFM) is an important tool for studying the dynamic behavior of large biomolecular assemblies at surfaces....
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High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy
A critical requirement for studying cell mechanics is three-dimensional assessment of cellular shapes and forces with high spatiotemporal resolution....
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MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological map** of live cells
Map** 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such...
