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The Ku complex: recent advances and emerging roles outside of non-homologous end-joining
Since its discovery in 1981, the Ku complex has been extensively studied under multiple cellular contexts, with most work focusing on Ku in terms of...
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The molecular basis and disease relevance of non-homologous DNA end joining
Non-homologous DNA end joining (NHEJ) is the predominant repair mechanism of any type of DNA double-strand break (DSB) during most of the cell cycle...
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Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica
Genomic variants libraries are conducive to obtain dominant strains with desirable phenotypic traits. The non-homologous end joining (NHEJ), which...
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Mechanism, cellular functions and cancer roles of polymerase-theta-mediated DNA end joining
Cellular pathways that repair chromosomal double-strand breaks (DSBs) have pivotal roles in cell growth, development and cancer. These DSB repair...
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Keap1 inhibition sensitizes head and neck squamous cell carcinoma cells to ionizing radiation via impaired non-homologous end joining and induced autophagy
The function of Keap1 (Kelch-like ECH-associated protein 1), a sensor of oxidative and electrophilic stress, in the radiosensitivity of cancer cells...
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Constructing founder sets under allelic and non-allelic homologous recombination
Homologous recombination between the maternal and paternal copies of a chromosome is a key mechanism for human inheritance and shapes population...
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Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks
BackgroundCRISPR-Cas9 genome editing often induces unintended, large genomic rearrangements, posing potential safety risks. However, there are no...
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Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Nonhomologous End Joining
This book chapter describes a plasmid-based reporter method, first described by Bennardo et al. (2008) that we use in our laboratory for determining...
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Alternative end-joining originates stable chromosome aberrations induced by etoposide during targeted inhibition of DNA-PKcs in ATM-deficient tumor cells
ATM and DNA-PKcs coordinate the DNA damage response at multiple levels following the exposure to chemotherapy. The Topoisomerase II poison etoposide...
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Molecular breeding of sporeless strains of Pleurotus ostreatus using a non-homologous DNA end-joining defective strain
Gene targeting is useful to isolate strains with mutations in a gene of interest for efficient breeding. In this study, we generated msh4 or mer3 ...
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POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis
BRCA2-mutant cells are defective in homologous recombination, making them vulnerable to the inactivation of other pathways for the repair of DNA...
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Non-homologous chromosome pairing during meiosis in haploid Brassica rapa
Key messageCytological observations of chromosome pairing showed that evolutionarily genome duplication might reshape non-homologous pairing during...
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A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins
DNA double-strand breaks (DSBs) are mainly repaired by homologous recombination (HR) and non-homologous end joining (NHEJ). The choice of HR or NHEJ...
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ASTE1 promotes shieldin-complex-mediated DNA repair by attenuating end resection
The shieldin complex functions as the downstream effector of 53BP1–RIF1 to promote DNA double-strand break end-joining by restricting end resection....
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DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres
Telomeres replicated by leading-strand synthesis lack the 3′ overhang required for telomere protection. Surprisingly, resection of these blunt...
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The deubiquitinase OTUD5 regulates Ku80 stability and non-homologous end joining
The ability of cells to repair DNA double-strand breaks (DSBs) is important for maintaining genome stability and eliminating oncogenic DNA lesions....
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Di- and tri-methylation of histone H3K36 play distinct roles in DNA double-strand break repair
Histone H3 Lys36 (H3K36) methylation and its associated modifiers are crucial for DNA double-strand break (DSB) repair, but the mechanism governing...
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cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence
As a sensor of cytosolic DNA, the role of cyclic GMP-AMP synthase (cGAS) in innate immune response is well established, yet how its functions in...
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Dynamics of the DYNLL1–MRE11 complex regulate DNA end resection and recruitment of Shieldin to DSBs
The extent and efficacy of DNA end resection at DNA double-strand breaks (DSB) determine the repair pathway choice. Here we describe how the...
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Genome sequencing of evolved aspergilli populations reveals robust genomes, transversions in A. flavus, and sexual aberrancy in non-homologous end-joining mutants
BackgroundAspergillus spp. comprises a very diverse group of lower eukaryotes with a high relevance for industrial applications and clinical...
