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Studying Secretory Protein Synthesis in Nicotiana benthamiana Protoplasts
Transient gene expression in plant protoplasts facilitates the analysis of hybrid genes in a fast and reproducible manner. The technique is...
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Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration
Insertion of a specific sequence in a targeted region for precise editing is still a major challenge in plants. Current protocols rely on inefficient...
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Application of Protoplast Regeneration to CRISPR /Cas9 Mutagenesis in Nicotiana tabacum
Protoplast transfection is widely used in plant research to rapidly evaluate RNA degradation, reporter assay, gene expression, subcellular...
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Experimental Interspecific Hybrids in Nicotiana and Barriers to Hybridization
The history of interspecific hybridization in Nicotiana is reviewed, starting with the work of Koelreuter who in the eighteenth century produced a...
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Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research
Protoplasts are the naked plant cells lacking the rigid cell wall and have been broadly utilized as an excellent tool to study the molecular...
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Establishment of protoplasts transient expression system in Pinellia ternata (Thunb.) Breit
ObjectiveIn this study, we established an efficient and rapid transient expression system in the protoplasts of Pinellia ternata (Thunb.) Breit. ( P....
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Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing
The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and...
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Experimental Introgression from Nicotiana Species to Cultivated Tobacco
A number of breeding goals and strategies involving the use of different Nicotiana species can be envisaged. Yet it is only breeding for resistance...
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Isolation, culture of Platycodon grandiflorus protoplasts: factors affecting protoplast yield, cell division, and micro-callus formation
Plyatocodon grandiflorus is an important medicinal herb, its rhizomes are utilized as vegetables and as medicinal food. In this investigation, we...
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Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf
Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting...
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Pooled effector library screening in protoplasts rapidly identifies novel Avr genes
Crop breeding for durable disease resistance is challenging due to the rapid evolution of pathogen virulence. While progress in resistance ( R ) gene...
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Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling
BackgroundSalsola laricifolia is a typical C 3 –C 4 typical desert plant, belonging to the family Amaranthaceae . An efficient single-cell system is...
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A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology
Nicotiana benthamiana is an invaluable model plant and biotechnology platform with a ~3 Gb allotetraploid genome. To further improve its usefulness...
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Efficiency of Transient Expression in Protoplasts of Various Potato Cultivars
AbstractThe production of mesophilic protoplasts of potato ( Solanum tuberosum L.) for transient gene expression is a required technological stage in...
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CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration
Clustered regularly interspaced short palindromic repeats (CRISPRCRISPR)-Cas (CRISPR-associated system) has become the multipurpose tool to...
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Transfection of Barley Leaf Protoplasts with a Fluorescently Tagged Fungal Effector for In Planta Localization Studies in Barley
The isolation and transfection of protoplasts from plant leaves have been routinely used for transient expression and functional studies in model...
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Isolation and Visualization of Plant Stress Granule-Associated Components via On-Beads Digestion and Co-localization Analysis
Stress granules (SGs) are conserved cytoplasmic biomolecular condensates mainly formed by proteins and RNA molecules assembled by liquid-liquid phase...
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Isolation, chemical fusion, and culture systems for olive (Olea europaea L.) protoplasts
Oleasters are olive genotypes that range from wild to feral. They are tolerant to biotic and abiotic stresses and are easily propagated. This study...
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Nicotiana hairy roots for recombinant protein expression, where to start? A systematic review
BackgroundHairy roots are a plant-tissue culture raised by Rhizobium rhizogenes infection (formerly known as Agrobacterium rhizogenes ). Nowadays,...
