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1. sRNA Structural Modeling Based on NMR Data

   Small non-coding RNAs (sRNAs) play vital roles in gene expression regulation and RNA interference. To comprehend their molecular mechanisms and...

   Pengzhi Wu, Lingna Yang in Bacterial Regulatory RNA

   Protocol 2024
   

2. Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate

   Besides the historical and traditional use of nuclear magnetic resonance (NMR) spectroscopy as a structure elucidation tool for proteins and...

   Caroline Mauve, Céline Oury, ... Michael Hodges in Photorespiration

   Protocol 2024
   

3. Solution NMR Analysis of O-Glycopeptide–Antibody Interaction

   O-Linked glycans potentially play a functional role in cellular recognition events. Recent structural analyses suggest that O-glycosylation can be a...

   Ryoka Kokubu, Shiho Ohno, ... Yoshiki Yamaguchi in Mucins

   Protocol 2024
   

4. Structure of prolylrapamycin: confirmation through a revised and detailed NMR assignment study

   A complete and detailed characterization of Rapamycin ( 1 ) and Prolylrapamycin ( 2 ) has been conducted by homo- and hetero-nuclear NMR experiments in...

   Annalisa Mortoni, Eugenio Castelli, ... Paolo Quadrelli in The Journal of Antibiotics

   Article Open access 19 March 2024
   

5. Improving quality of analysis by suppression of unwanted signals through band-selective excitation in NMR spectroscopy for metabolomics studies

   Introduction

   Nuclear Magnetic Resonance (NMR) spectroscopy stands as a preeminent analytical tool in the field of metabolomics. Nevertheless, when it...

   Upendra Singh, Ruba Al-Nemi, ... Mariusz Jaremko in Metabolomics

   Article 19 December 2023
   

6. Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on 15N-Labelled Ammonium Ions

   Solid-state NMR allows for the study of membrane proteins under physiological conditions. Here we describe a method for detection of bound ions in...

   Carl Öster, Sascha Lange, ... Adam Lange in Potassium Channels

   Protocol 2024
   

7. NMR Analysis of the Interactions and Conformational Plasticity of Dynein Intermediate Chain

   Cytoplasmic dynein complexes play crucial roles in intracellular transport of cellular organelles. While the motor domain of dynein is well...

   Kayla A. Jara, Elisar J. Barbar in Dynein

   Protocol 2023
   

8. The conformational properties of alamethicin in ethanol studied by NMR

   Alamethicin, a peptide consisted of 20 amino acid residues, has been known to function as an antibiotic. The peptides self-associate in biological...

   Yoshinori Miura in European Biophysics Journal

   Article 08 June 2024
   

9. CP MAS ¹³C NMR Spectroscopy in Determination of Species-Specific Differences in Wood Composition

   Abstract

   In recent years, solid-state ¹³ C NMR spectroscopy using the techniques of cross-polarization (CP) and magic angle spinning (MAS) has been...

   S. G. Kostryukov, P. S. Petrov, ... N. S. Kostryukov in Russian Journal of Bioorganic Chemistry

   Article 28 December 2022
   

10. Rapid reaction optimization by robust and economical quantitative benchtop ¹⁹F NMR spectroscopy

    The instrumental analysis of reaction mixtures is usually the rate-determining step in the optimization of chemical processes. Traditionally,...

    G. Heinrich, M. Kondratiuk, ... M. P. Wiesenfeldt in Nature Protocols

    Article 26 February 2024
    

11. NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes

    Nuclear magnetic resonance (NMR) spectroscopy is the method of choice for studying the dynamics of biological macromolecules in solution. By...

    Michelangelo Marasco, John P. Kirkpatrick, ... Teresa Carlomagno in SH2 Domains

    Protocol 2023
    

12. A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats

    Abstract

    Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent...

    Jorge Luis Nicoleti, Erik Sobrinho Braga, ... Ricardo Wagner Portela in Applied Microbiology and Biotechnology

    Article 23 May 2023
    

13. Performance evaluate of different chemometrics formalisms used for prostate cancer diagnosis by NMR-based metabolomics

    Introduction

    In general, two characteristics are ever present in NMR-based metabolomics studies: (1) they are assays aiming to classify the samples in...

    Márcio Felipe Oliveira, Moacir Cavalcante de Albuquerque Neto, ... Ricardo Oliveira Silva in Metabolomics

    Article 21 December 2023
    

14. Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy

    High-resolution structural studies of proteins and protein complexes in a native eukaryotic environment present a challenge to structural biology....

    Nicholas Sciolino, Anna Liu, ... Alexander Shekhtman in Communications Biology

    Article Open access 12 May 2022
    

15. NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows

    Introduction

    Absolute quantification of individual metabolites in complex biological samples is crucial in targeted metabolomic profiling.

    ...

    Cécile Canlet, Catherine Deborde, ... Patrick Giraudeau in Metabolomics

    Article Open access 07 July 2023
    

16. NMR spectroscopy of lipidic cubic phases

    Lipidic cubic phase (LCP) structures have been used for stabilisation and crystallisation of membrane proteins and show promising properties as drug...

    Sunnia Rajput, Shenggen Yao, ... Frances Separovic in Biophysical Reviews

    Article 11 November 2021
    

17. Problems, principles and progress in computational annotation of NMR metabolomics data

    Background

    Compound identification remains a critical bottleneck in the process of exploiting Nuclear Magnetic Resonance (NMR) metabolomics data,...

    Michael T. Judge, Timothy M. D. Ebbels in Metabolomics

    Article Open access 05 December 2022
    

18. Solution NMR Spectroscopy for Characterizing Protein–Glycosaminoglycan Interactions

    Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited...

    Prem Raj B. Joseph, Krishna Mohan Sepuru, ... Krishna Rajarathnam in Glycosaminoglycans

    Protocol 2022
    

19. Hyperpolarized water as universal sensitivity booster in biomolecular NMR

    NMR spectroscopy is the only method to access the structural dynamics of biomolecules at high (atomistic) resolution in their native solution state....

    Christian Hilty, Dennis Kurzbach, Lucio Frydman in Nature Protocols

    Article 11 May 2022
    

20. NMR techniques in studying water in biotechnological systems

    Different NMR methodologies have been considered in studying water as a part of the structure of heterogeneous biosystems. The current work mostly...

    Victor V. Rodin in Biophysical Reviews

    Article 15 June 2020