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A Novel Multiplex RT-PCR for Simultaneous Detection of Malaria, Chikungunya and Dengue Infection (MCD-RT-PCR)
The “triple whammy” of infection of Malaria, Chikungunya and Dengue can considerably change the spectrum of clinical manifestations that complicates...
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Multiplex RT-PCR
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful...
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Simultaneous detection and differentiation of four viruses in passion fruit plants by a multiplex RT-PCR
A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) assay was developed to enable the simultaneous detection and...
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Tube-Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees
Diagnosis of fruit tree viruses has been challenging for a long time as viral titer is often low and unevenly distributed among different tissues and...
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Development of one-step multiplex RT-PCR assay for rapid simultaneous detection of five RNA viruses and Acidovorax citrulli in major cucurbitaceous crops in China
Cucurbitaceous fruits and vegetables are important crops. Viral and bacterial diseases cause substantial economic losses to cucurbit crops globally....
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Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens
AbstractA prototype of a system for the detection of infectious human pneumonia pathogens based on multiplex solid-phase reverse transcription PCR...
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Integrated point-of-care RT-PCR methods during and after COVID-19 pandemic
The COVID-19 pandemic has taken the world by surprise and people and organisations worldwide worked in some way or the other to combat the spread;...
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Use of dual priming oligonucleotide system-based multiplex RT-PCR assay to detect five diarrhea viruses in pig herds in South China
In this study, a specific and simple method based on the dual priming oligonucleotide (DPO) system was developed to simultaneously detect...
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One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries...
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Development of multiplex allele-specific RT-qPCR assays for differentiation of SARS-CoV-2 Omicron subvariants
AbstractQuick differentiation of current circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their...
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Next-generation sequencing identification and multiplex RT-PCR detection for viruses infecting cigar and flue-cured tobacco
BackgroundEarly, precise and simultaneous identification of plant viruses is of great significance for preventing virus spread and reducing losses in...
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Comparison of pneumococcal colonization density among healthy children and children with respiratory symptoms using real time PCR (RT-PCR)
BackgroundNasopharyngeal colonization is considered a necessary step in the initiation of pneumococcal diseases. Real time PCR (RT-PCR) is an...
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RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
For the past 30 years, in vitro transcription (IVT) technology has been extensively used for RNA production or for basic transcriptional mechanism...
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TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice
Members of PARP family are responsible for poly(ADP-ribose) (pADPr) posttranslational modification synthesis. They are intensively studied proteins...
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Detection of Viral Agents Causing Gastroenteritis by Multiplex PCR Method in Patients; Norovirus, Rotavirus, Adenovirus, Astrovirus, Sapovirus
Acute viral gastroenteritis may lead hospitalization and death in individuals with underlying health conditions such as immunosuppression....
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Simultaneous Detection of Grapevine Viruses via Multiplex Reverse Transcription Polymerase Chain Reaction: An Academic Approach
Grapevine viruses pose a significant threat to the viticulture industry, resulting in reduced grape yield, poor fruit quality, and economic loss....
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Presence and localization of apelin and its cognate receptor in canine testes using immunohistochemical and RT-PCR techniques
Apelin, a member of the adipokine family, is a novel endogenous peptide which regulates the male reproductive system of mammals by interacting with a...
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Multiplex Detection of RNA Viruses Based on Ligation Reaction and Universal PCR Amplification
To detect several RNA viruses simultaneously, a method based on multiplex ligation reaction combined with multiplex qPCR or multiplex PCR+capillary...
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Design and Evaluation of Multiplex One-Step Reverse Transcription PCR–Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens
Influenza A, influenza B, severe acute respiratory syndrome coronavirus 2, adenovirus, respiratory syncytial virus, Mycoplasma pneumoniae , and Chlamydo...
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Simultaneous detection of citrus concave gum-associated virus (CCGaV) and citrus virus A (CiVA) by multiplex RT-PCR
Citrus concave-gum associated virus (CCGaV) and citrus virus A (CiVA) are two negative-stranded RNA viruses infecting citrus. Both viruses have been...
