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Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification
BackgroundMycobacterium leprae (ML) is the pathogen that causes leprosy, which has a long history and still exists today. ML is an intracellular...
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Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes
BackgroundS. pyogenes , is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and...
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Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor
BackgroundBurkholderia pseudomallei ( B. pseudomallei ), as a highly pathogenic organism, causes melioidosis, which is a disease of public health...
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Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila
BackgroundLegionella pneumophila is an opportunistic waterborne pathogen of significant public health problems, which can cause serious human...
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Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex
BackgroundTuberculosis (TB) is a serious chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC). Hence, the development of a...
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Visual multiple cross displacement amplification for the rapid identification of S. agalactiae immediately from vaginal and rectal swabs
Streptococcus agalactiae ( S. agalactiae ) is an important pathogen that can lead to neonatus and mother infection. The current existing techniques for...
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Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases
Plant pathogens cause severe losses to crop yields and economic returns in agriculture. Despite plant tissue DNA extraction of typically constituting...
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Identification of Circular RNAs by Multiple Displacement Amplification and Their Involvement in Plant Development
With the innovative knowledge and bioinformatics tools in the identification and characterization of noncoding RNAs, circular RNA (circRNA) is added...
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Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis
BackgroundBordetella pertussis is the causative agent of pertussis, a respiratory tract infectious disease .
Efficient techniques for detection of B....
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Isothermal exponential amplification reactions triggered by circular templates (cEXPAR) targeting miRNA
BackgroundIsothermal exponential amplification reaction (EXPAR) is an emerging amplification technique that is most frequently used to amplify...
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Rapid detection of chilli leaf curl virus using loop-mediated isothermal amplification
Chilli leaf curl virus (ChiLCV) is a significant begomovirus that infects chili plants. To detect ChiLCV infection, a loop-mediated isothermal...
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LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA
Methylation-based liquid biopsies show promises in detecting cancer using circulating cell-free DNA; however, current limitations impede clinical...
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Tetra-Primer Amplification Refractory Mutation System (T-ARMS)
Single-nucleotide polymorphisms (SNPs), the most abundant genetic variation in the population, have become the molecular marker of choice. Generally,...
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Rolling circle amplification (RCA)-based DNA hydrogel
DNA hydrogels have unique properties, including sequence programmability, precise molecular recognition, stimuli-responsiveness, biocompatibility and...
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Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification
Mycoplasma contamination in cell culture affects the properties of cell lines. Gold standard detection by microbiological culture takes days and...
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Strange Tunes—Acoustic Variation and Character Displacement in a Tarsier Hybrid Zone
Natural hybridization provides valuable insights into evolutionary processes, such as speciation and the forces driving or hindering it. Sulawesi...
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Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses
Loop-mediated isothermal amplification (LAMP) is a sensitive method that can rapidly amplify a specific nucleic acid target with high specificity....
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A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus
Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is...
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Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis
Syphilis is a re-emerging sexually transmitted disease caused by the pathogenic spirochete T. pallidum. Every year more than 5 million cases are...
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Rapid and quantitative detection of Aspergillus niger Van Tieghem using loop-mediated isothermal amplification assay
Peanut ( Arachis hypogaea L.) crown rot and root rot are common diseases caused by Aspergillus niger Van Tieghem. Early and accurate detection of A....
