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Computational Protocol for DNA Methylation Profiling in Plants Using Restriction Enzyme-Based Genome Reduction
Epigenetics can be described as heritable phenotype changes that do not involve alterations in the underlying DNA sequence. Having widespread...
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Reduced Representation Bisulfite Sequencing (RRBS)
Reduced representation bisulfite sequencing (RRBS) enriches CpG-rich genomic regions using the MspI restriction enzyme—which cuts DNA at all CCGG...
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Strain Identification and Herbivore–Host Interaction of Maize Hosting Invasive Alien Pest Spodoptera frugiperda under Elevated Temperature and CO2 Levels
The fall armyworm, Spodoptera frugiperda is a recent invasive significant pest of maize in India for the past three years. As, there are two strains...
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3D-GBS: a universal genoty**-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes
Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing...
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Estimation of Heterografting Associated DNA Methylation Changes in Tree Crops by MSAP Analysis
The genetic incompatibility of the seedlings which are used as rootstocks (stock–scion interactions) and the mechanical stress induced by grafting...
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Genome-wide SNP discovery in native American and Hungarian Robinia pseudoacacia genotypes using next-generation double-digest restriction-site-associated DNA sequencing (ddRAD-Seq)
Robinia pseudoacacia L. (commonly known as black locust) is an economically and environmentally important plant, native to the eastern USA, and...
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Effects of maternal methyl donor intake during pregnancy on ileum methylation and function in an intrauterine growth restriction pig model
BackgroundIntrauterine growth retardation (IUGR) affects intestinal growth, morphology, and function, which leads to poor growth performance and high...
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Diversity of individuals' methylation patterns to different moisture regimes in Einkorn wheat revealed by CRED-RA technique
Diploid species with desirable traits among wheat's wild relatives have a high potential for crop modification. DNA methylation is one of the...
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Association of xenobiotic-metabolizing genes polymorphisms with cervical cancer risk in the Tunisian population
BackgroundHost genetic characteristics and environmental factors interactions may play a crucial role in cervical carcinogenesis. We investigated the...
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Investigation of antifungal activities of myrcene on Fusarium reference strains
Antifungal effects of myrcene, the plant-based naturel compound, were investigated on Fusarium graminearum PH-1 and Fusarium culmorum FcUK99...
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DNA Cutters in Recombinant DNA Technology
Restriction enzymes are special types of endonucleases that have become a very valuable tool to the molecular biologist. These are a part of the...
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Effects of mammalian sex hormones on regeneration capacity, retrotransposon polymorphism and genomic instability in wheat (Triticum aestivum L.)
Mammalian steroid hormones (MSH) are also inherently synthesized by several plant species. However, external application of steroid hormones further...
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Genoty**-by-Sequencing (GBS ) Method for Accelerating Marker-Assisted Selection (MAS) Program
Marker-assisted selection (MAS) plays a pivotal role in a breeding program where molecular DNA markers are used for phenotypic selections in crop...
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Bringing to light the preponderance and origin of spontaneous triploid cytotypes in medicinal Tinospora cordifolia: implications for genetic improvement
Tinospora cordifolia ( Giloy), with immense therapeutic properties has been used as a powerful rasayana and immunomodulator in alternative systems of...
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Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
BackgroundThe generation of induced pluripotent stem cells has opened the field of study for stem cell research, disease modeling and drug...
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Growth, physiological, biochemical and DNA methylation responses to cadmium stress of bean (phaseolus vulgaris L) grown under different irrigation levels
The aim of this study is to evaluate the physiological, biochemical and morphological characteristics of bean in different irrigation (I1: 100%, I2:...
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Population Genomics Analysis with RAD, Reprised: Stacks 2
Restriction enzymes have been one of the primary tools in the population genetics toolkit for 50 years, being coupled with each new generation of...
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Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing
BackgroundGenome-wide data are invaluable to characterize differentiation and adaptation of natural populations. Reduced representation sequencing...
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Isolation of Pseudomonas syringae pv. Tomato strains causing bacterial speck disease of tomato and marker-based monitoring for their virulence
BackgroundThe bacterial speck disease of tomato caused by a bacterial pathogen Pseudomonas syringae pv. tomato is a most important disease causing...
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Comparison of genoty** by sequencing procedures to determine population genetic structure
Advancements in technology over the past few decades have resulted in the development of genome sequencing at lower costs. Protocols, costs, and the...
