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Targeted Mutagenesis
Mutational analysis in forward genetics is an effective strategy in invertebrate model animals and zebrafish in vertebrates. Forward genetic studies...
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Loss-of-Function Mutagenesis for Forward Genetics
Genes are central players regulating biological processes. A general strategy for studying gene function is to impair gene activity and examine its...
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Transposable Elements II: Insertional Mutagenesis
This chapter is a sequel to the topic of transposable elements discussed in Chap. 5 . Here, we will...
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Asian Mouse Mutagenesis Resource Association (AMMRA): mouse genetics and laboratory animal resources in the Asia Pacific
The Asian Mouse Mutagenesis Resource Association (AMMRA) is a non-profit organization consisting of major resource and research institutions with...
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Pan-drug resistance and hypervirulence in a human fungal pathogen are enabled by mutagenesis induced by mammalian body temperature
The continuing emergence of invasive fungal pathogens poses an increasing threat to public health. Here, through the China Hospital Invasive Fungal...
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Alternative Mechanisms of Mutagenesis at mCpG Sites during Replication and Repair
Abstract5-Methyl-2'-deoxycytidine (mC) at CpG sites plays a key role in the epigenetic gene regulation, cell differentiation, and carcinogenesis....
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Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted...
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Revisiting mutagenesis at non-B DNA motifs in the human genome
Non-B DNA structures formed by repetitive sequence motifs are known instigators of mutagenesis in experimental systems. Analyzing this phenomenon...
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Computational Random Mutagenesis to Investigate RAS Mutant Signaling
This chapter describes how mathematical models can be used to investigate the possible range of behaviors for mutant forms of a protein. A...
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Targeted Mutagenesis in Mice Using a Base Editor
Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly...
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Targeted mutagenesis in mice via an engineered AsCas12f1 system
SpCas9 and AsCas12a are widely utilized as genome editing tools in human cells, but their applications are largely limited by their bulky size....
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Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2
Patients with idiopathic pulmonary fibrosis show a strongly upregulated expression of chemokine CXCL14, whose target is still unknown. Screening of...
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Mouse Models to Study Peroxisomal Functions and Disorders: Overview, Caveats, and Recommendations
During the last three decades many mouse lines were created or identified that are deficient in one or more peroxisomal functions. Different...
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Precancerous liver diseases do not cause increased mutagenesis in liver stem cells
Inflammatory liver disease increases the risk of develo** primary liver cancer. The mechanism through which liver disease induces tumorigenesis...
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Progress towards completing the mutant mouse null resource
The generation of a comprehensive catalog of null alleles covering all protein-coding genes is the goal of the International Mouse Phenoty**...
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Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System
Engineering of the mouse germline is a key technology in biomedical research for studying the function of genes in health and disease. Since the...
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Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus
The targeting of transgenic constructs at single copy into neutral genomic loci avoids the unpredictable outcomes associated with conventional random...
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Structure of human DPPA3 bound to the UHRF1 PHD finger reveals its functional and structural differences from mouse DPPA3
DNA methylation maintenance is essential for cell fate inheritance. In differentiated cells, this involves orchestrated actions of DNMT1 and UHRF1....
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Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes
Aneuploidy seriously compromises female fertility and increases incidence of birth defects. Rates of aneuploidy in human eggs from even young women...
