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MAIRA: Protein-based Analysis of MinION Reads on a Laptop
Third-generation sequencing technologies are being increasingly used in microbiome research and this has given rise to new challenges in...
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Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology
BackgroundAccurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although...
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Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION device
BackgroundSequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence...
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Sterile sentinels and MinION sequencing capture active soil microbial communities that differentiate crop rotations
BackgroundSoil microbial communities are difficult to measure and critical to soil processes. The bulk soil microbiome is highly diverse and...
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Fast Identification Method for Screening Bacteria from Faecal Samples Using Oxford Nanopore Technologies MinION Sequencing
Most bacterial identification methods require extensive culturing, strain purification and DNA extraction protocols. This leads to additional...
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Exploring S-RNase diversity in the Andean black cherry (Prunus serotina) using MinION sequencing: a cost-effective approach with increased genoty** resolution
The Andean black cherry ( P. serotina ) is an underutilized fruit species that could contribute to the development of sustainable food systems in the...
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High quality genomes produced from single MinION flow cells clarify polyploid and demographic histories of critically endangered Fraxinus (ash) species
With populations of threatened and endangered species declining worldwide, efforts are being made to generate high quality genomic records of these...
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ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone
BackgroundDNA barcodes are a useful tool for discovering, understanding, and monitoring biodiversity which are critical tasks at a time of rapid...
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Long-amplicon MinION-based sequencing study in a salt-contaminated twelfth century granite-built chapel
AbstractThe irregular damp dark staining on the stonework of a salt-contaminated twelfth century granite-built chapel is thought to be related to a...
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Identification of additional fire blight resistance candidate genes following MinION Oxford Nanopore sequencing and assembly of BAC clone spanning the Malus fusca resistance locus
Pyramiding different fire blight resistance genes and QTLs in future apple cultivars is the most eco-friendly way to combat this disease....
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Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
Healthy plants are vital for successful, long-duration missions in space, as they provide the crew with life support, food production, and...
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Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution
BackgroundSpecies-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and...
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Genomic and resistome analysis of Alcaligenes faecalis strain PGB1 by Nanopore MinION and Illumina Technologies
BackgroundDrug-resistant bacteria are important carriers of antibiotic-resistant genes (ARGs). This fact is crucial for the development of precise...
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Scalable, Cost-Effective, and Decentralized DNA Barcoding with Oxford Nanopore Sequencing
DNA barcodes are useful in biodiversity research, but sequencing barcodes with dye termination methods (“Sanger sequencing”) has been so...
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Evaluation of NGS DNA barcoding for biosecurity diagnostic applications: case study from banana freckle incursion in Australia
Molecular diagnostics in combination with morphological identification is the method of choice for several cryptic microbial plant pathogens. For...
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High quality genome assemblies of Mycoplasma bovis using a taxon-specific Bonito basecaller for MinION and Flongle long-read nanopore sequencing
BackgroundImplementation of Third-Generation Sequencing approaches for Whole Genome Sequencing (WGS) all-in-one diagnostics in human and veterinary...
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Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens
The MinION sequencing platform offers near real-time analysis of DNA sequence; this makes the tool attractive for deployment in fieldwork or clinical...
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Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing
BackgroundMore than 80% of all animal species remain unknown to science. Most of these species live in the tropics and belong to animal taxa that...
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Nanopore Sequencing Techniques: A Comparison of the MinKNOW and the Alignator Sequencers
Whole genome and transcriptome analyses represent powerful tools. Despite improvements in sequencing methodology, whole transcriptome analyses are...
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The regulatory role of Myomaker and Myomixer–Myomerger–Minion in muscle development and regeneration
Skeletal muscle plays essential roles in motor function, energy, and glucose metabolism. Skeletal muscle formation occurs through a process called...
