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Growing and making nano- and microcrystals
Thanks to recent technological advances in X-ray and micro-electron diffraction and solid-state NMR, structural information can be obtained by using...
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Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments
Serial femtosecond crystallography (SFX) experiments using an X-ray free electron laser (XFEL) is a burgeoning method for time-resolved structural...
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Hypothetical Pathway for Formation of Cholesterol Microcrystals Initiating the Atherosclerotic Process
Major factors leading to the development of atherosclerosis are a high cholesterol (Chol) level in the blood and oxidative stress. Both promote the...
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Regulation of light energy conversion between linear and cyclic electron flow within photosystem II controlled by the plastoquinone/quinol redox poise
Ultrapurified Photosystem II complexes crystalize as uniform microcrystals (PSIIX) of unprecedented homogeneity that allow observation of details...
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Crystallization of Microbial Rhodopsins
Microbial rhodopsins are light-sensitive transmembrane proteins, evolutionary adapted by various organisms like archaea, bacteria, simple eukaryote,...
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Ab initio phasing macromolecular structures using electron-counted MicroED data
Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct...
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Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction
Changes in lattice structure across sub-regions of protein crystals are challenging to assess when relying on whole crystal measurements. Because of...
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Structural Analyses of Bacterial Effectors by X-Ray Crystallography
X-ray crystallography is a method of choice to determine and analyze protein structures. Although large complexes are challenging to crystallize and...
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Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography
Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales....
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Visualizing drug binding interactions using microcrystal electron diffraction
Visualizing ligand binding interactions is important for structure-based drug design and fragment-based screening methods. Rapid and uniform soaking...
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NETosis: Molecular Mechanisms, Role in Physiology and Pathology
AbstractNETosis is a program for formation of neutrophil extracellular traps (NETs), which consist of modified chromatin decorated with bactericidal...
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Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates
Time-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA....
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Structural Characterization of Multienzyme Assemblies: An Overview
Multienzyme assemblies have attracted significant attention in recent years for use in industrial applications instead of single enzymes. Owing to...
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Sucrose Recovery from Diluted Cane Molasses by Using Mass Transfer to the Third Liquid Layer at Contacting with Ethanol
A simple rate-based method is proposed for recovering sucrose from sugarcane molasses, focusing on its feasibility for continuous recovery. When...
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Virus Detection
For the observation of individualized viral particles, whose size is on the order of nanometers, analysis under a transmission electron microscope...
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Immunostaining for Epigenetic Modifications in Fagopyrum Calli
Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single...
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Crystallographic Studies of Rhodopsins: Structure and Dynamics
Crystal structures have provided detailed insight in the architecture of rhodopsin photoreceptors. Of particular interest are the protein–chromophore...
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Macromolecular movies, storybooks written by nature
Twelve years ago, the first free electron laser for hard X-rays (XFEL), the Linac Coherent Light Source (LCLS), came online. Due to the extreme...
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Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources
Macromolecular crystallography (MX) leverages the methods of physics and the language of chemistry to reveal fundamental insights into biology. Often...
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Rational Design of Drugs Targeting G-Protein-Coupled Receptors: A Structural Biology Perspective
AbstractG protein-coupled receptors (GPCRs) play a key role in the transduction of extracellular signals to cells and regulation of many biological...
