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Metabuli: sensitive and specific metagenomic classification via joint analysis of amino acid and DNA
Metagenomic taxonomic classifiers analyze either DNA or amino acid (AA) sequences. Metabuli ( https://metabuli.steineggerlab.com ), however, jointly...
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A Method of DNA Extraction from Plants for Metagenomic Analysis Based on the Example of Grape Vitis amurensis Rupr.
A new method for extracting DNA from plants is proposed, using the example of wild grapes Vitis amurensis Rupr. for further preparation of libraries...
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Construction of Small-Insert and Large-Insert Metagenomic Libraries
The vast majority of the Earth’s biological diversity are hidden in uncultured and yet uncharacterized microbial genomes. The construction of...
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Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems....
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Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods
BackgroundShotgun metagenomic sequencing has greatly expanded the understanding of microbial communities in various biological niches. However, it is...
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Recent Advances in Metagenomic Approaches, Applications, and Challenges
Advances in metagenomics analysis with the advent of next-generation sequencing have extended our knowledge of microbial communities as compared to...
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Effect of bacterial DNA enrichment on detection and quantification of bacteria in an infected tissue model by metagenomic next-generation sequencing
Before implementing metagenomic next-generation sequencing (mNGS) in the routine diagnostic laboratory, several challenges need to be resolved. To...
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Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production
The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene...
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aMeta: an accurate and memory-efficient ancient metagenomic profiling workflow
Analysis of microbial data from archaeological samples is a growing field with great potential for understanding ancient environments, lifestyles,...
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Interactive Web-Based Services for Metagenomic Data Analysis and Comparisons
Recently, sequencing technologies have become readily available, and scientists are more motivated to conduct metagenomic research to unveil the...
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Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes
Here, we outline how to identify hydrogenase enzymes from metagenomic fosmid libraries through an activity-based screening approach. A metagenomic...
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Improved method for the extraction of high-quality DNA from lignocellulosic compost samples for metagenomic studies
AbstractThe world economy is currently moving towards more sustainable approaches. Lignocellulosic biomass has been widely used as a substitute for...
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Metagenomic sequencing reveals swine lung microbial communities and metagenome-assembled genomes associated with lung lesions—a pilot study
Low microbial biomass in the lungs, high host-DNA contamination and sampling difficulty limit the study on lung microbiome. Therefore, little is...
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Bioremediation of Heavy Metals by Metagenomic Approaches
Deposition of heavy metals and other contaminating materials in the environment is a ceaseless and inescapable process. Bioaccumulation of heavy...
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Integration of polygenic and gut metagenomic risk prediction for common diseases
Multiomics has shown promise in noninvasive risk profiling and early detection of various common diseases. In the present study, in a prospective...
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A new and efficient enrichment method for metagenomic sequencing of Monkeypox virus
BackgroundThe methodology described in previous literature for Monkeypox virus (MPXV) sequencing shows low efficiency when using metagenomic...
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Construction and Functional Screening of Metagenomic Libraries for D-Stereospecific Amidases and Their Catalytic Promiscuity
AbstractMetagenomic fosmid libraries were prepared from soil and water samples collected from diverse places. Clones in the range of (1.1–2.8) × 10 3 ...
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Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System
Metagenomic screening is a widely applied biotechnological approach for screening of novel industrial enzymes. The traditional method of metagenomic...
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Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing
The plant microbiome is crucial for plant growth, yet many important questions remain, such as the identification of specific bacterial species in...
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Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
AbstractCheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein...
