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Membrane Trafficking Methods and Protocols
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in...
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Generation of nanoscopic membrane curvature for membrane trafficking
Curved membranes are key features of intracellular organelles, and their generation involves dynamic protein complexes. Here we describe the...
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Electrostatic switch mechanisms of membrane protein trafficking and regulation
Lipid-protein interactions are normally classified as either specific or general. Specific interactions refer to lipid binding to specific binding...
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N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope
Fibroblast growth factor receptor 1 (FGFR1) is a heavily N-glycosylated cell surface receptor tyrosine kinase that transmits signals across the...
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GOLGA7 is essential for NRAS trafficking from the Golgi to the plasma membrane but not for its palmitoylation
NRAS mutations are most frequently observed in hematological malignancies and are also common in some solid tumors such as melanoma and colon cancer....
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EDA ligand triggers plasma membrane trafficking of its receptor EDAR via PKA activation and SNAP23-containing complexes
BackgroundEctodysplasin-A (EDA), a skin-specific TNF ligand, interacts with its membrane receptor EDAR to trigger EDA signaling in skin appendage...
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A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are...
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Sterol Endocytosis and Trafficking in Plant Cells
Structural sterols are integral components of biological membranes. They regulate membrane permeability and fluidity, and they influence the...
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The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium
The efficient transport of proteins into the primary cilium is a crucial step for many signaling pathways. Dysfunction of this process can lead to...
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Identified and potential internalization signals involved in trafficking and regulation of Na+/K+ ATPase activity
The sodium–potassium pump (NKA) or Na + /K + ATPase consumes around 30–40% of the total energy expenditure of the animal cell on the generation of the...
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Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing
Vesicle trafficking entails packaging and transport of membrane-associated proteins to their target membranes, and recycling or degradation of...
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A Rab10–ACAP1–Arf6 GTPases cascade modulates M4 muscarinic acetylcholine receptor trafficking and signaling
Membrane trafficking processes regulate the G protein-coupled receptor activity. The muscarinic acetylcholine receptors (mAChRs) are highly pursued...
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Flotillins affect LPS-induced TLR4 signaling by modulating the trafficking and abundance of CD14
Lipopolysaccharide (LPS) induces a strong pro-inflammatory reaction of macrophages upon activation of Toll-like receptor 4 (TLR4) with the assistance...
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Differential intracellular trafficking of extracellular vesicles in microglia and astrocytes
Extracellular vesicles (EVs) have emerged as key players in cell-to-cell communication in both physiological and pathological processes in the...
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Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins
Light scattering methods permit the determination of molar mass and hydrodynamic radius for a protein from first principles. They are, therefore,...
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Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
Measuring the functional behavior of G protein-coupled receptors (GPCRs) has been a major focus of academic and pharmaceutical research for many...
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Cholesterol Hydroperoxide Co-trafficking in Testosterone-generating Leydig Cells: GPx4 Inhibition of Cytotoxic and Anti-steroidogenic Effects
Trafficking of intracellular cholesterol (Ch) to and into mitochondria of steroidogenic cells is required for steroid hormone biosynthesis. This...
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Introduction: Regulatory processes, an emerging feature in intracellular membrane traffic
The subject of this volume is the molecular mechanism of the intracellular membrane trafficking, a central eukaryotic cell biological process. In the...
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Real-time imaging of axonal membrane protein life cycles
The construction of neuronal membranes is a dynamic process involving the biogenesis, vesicular packaging, transport, insertion and recycling of...
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BECLIN1 is essential for intestinal homeostasis involving autophagy-independent mechanisms through its function in endocytic trafficking
Autophagy-related genes have been closely associated with intestinal homeostasis. BECLIN1 is a component of Class III phosphatidylinositol 3-kinase...
