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Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
We show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces...
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Map** Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotSSDS-extra
Molecular approaches are required to detect DNA double-strand break (DSB) events and to map and quantify them at high resolution. One of the most...
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Meiotic prophase roles of Pds5 in recombination and chromosome condensation in budding yeast
Genetic variation in eukaryotes is mediated during meiosis by the exchange of genetic material between homologous chromosomes to produce recombinant...
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Meiotic Instability Generates a Pathological Condition in Mammalian Ovum
Maintenance of metaphase-II (M-II) arrest in ovum is required to present itself as a right gamete for successful fertilization in mammals....
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Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages
PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other...
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Human SLFN5 and its Xenopus Laevis ortholog regulate entry into mitosis and oocyte meiotic resumption
The Schlafen gene family was first described in mice as a regulator of thymocyte development. Further studies showed involvement of human orthologs...
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Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining
In the adult mouse testis, germ cells of various developmental cell states co-exist. FACS isolation of cells stained with the DNA dye Hoechst 33342...
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Meiotic regulation of the Ndc80 complex composition and function
This review describes the current models for how the subunit abundance of the Ndc80 complex, a key kinetochore component, is regulated in budding...
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Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis
Transcriptomic analyses of germ cells at different stages of differentiation have shed light on the transcriptional and post-transcriptional...
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Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis
Whole-mount immunofluorescence allows direct visualization of the cellular architecture within cells. Here, we apply this technique to mouse oocytes...
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Transgenerational conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers
Key message Maize
Outer cell layer 4 (ocl4) encodes an HD-ZIP IV transcription factor required for robust male fertility and 21-nt phasiRNA biogenesis ...
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Systematic genetic and proteomic screens during gametogenesis identify H2BK34 methylation as an evolutionary conserved meiotic mark
BackgroundGametes are highly differentiated cells specialized to carry and protect the parental genetic information. During male germ cell...
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RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo
The maintenance of genome integrity in the germline is crucial for mammalian development. Long interspersed element type 1 (LINE-1, L1) is a mobile...
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The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division
Cell division is orchestrated by the phosphorylation and dephosphorylation of thousands of proteins. These post-translational modifications underlie...
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The DNA damage checkpoint and the spindle position checkpoint: guardians of meiotic commitment
Exogenous signals induce cells to enter the specialized cell division process of meiosis, which produces haploid gametes from diploid progenitor...
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Heritability of meiotic restitution and fertility restoration in haploid triticale
Key messageA single division meiosis mechanism of meiotic restitution is incompletely penetrant but significantly associated with restored fertility...
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Protein Kinases Involved in Mitotic Spindle Checkpoint Regulation
A number of checkpoint controls function to preserve the genome by restraining cell cycle progression until prerequisite events have been properly...
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Cytological and transcriptomic analyses provide insights into the pollen fertility of synthetic allodiploid Brassica juncea hybrids
Key messageImbalanced chromosomes and cell cycle arrest, along with down-regulated genes in DNA damage repair and sperm cell differentiation, caused...
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Asynapsis and unreduced gamete formation in a Trifolium interspecific hybrid
BackgroundUnreduced gametes, a driving force in the widespread polyploidization and speciation of flowering plants, occur relatively frequently in...
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Twenty years of merotelic kinetochore attachments: a historical perspective
Micronuclei, small DNA-containing structures separate from the main nucleus, were used for decades as an indicator of genotoxic damage. Micronuclei...
